癫痫伴发抑郁大鼠额前皮质脑源性神经营养因子及其受体的表达研究  

Expressions of brain-derived neurotrophic factor and its receptor in prefrontal cortex of rats with epilepsy-associated depression

作　　者：许新炜 杨钧哲 朱含笑 李漾超 李云 Xu Xinwei;Yang Junzhe;Zhu Hanxiao;Li Yangchao;Li Yun(Dali University School of Clinical Medicine,Dali 671000,Yunnan Province,China)

机构地区：[1]大理大学临床医学院,671000 [2]大理大学第一附属医院神经内科

出　　处：《中华老年心脑血管病杂志》2019年第1期77-81,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：云南省教育厅科学研究基金(2015Z163)

摘　　要：目的观察癫痫伴发抑郁(epilepsy-associated depression,EAD)大鼠额前皮质脑源性神经营养因子(brain-derived neurotrophic fctor,BDNF)及其高亲和力受体原肌球蛋白受体激酶B(tropomyosin receptor kinase B,Trk B)的表达情况。方法 52只雌性SD大鼠随机分为正常组、抑郁组(采用慢性不可预见中等应激刺激结合孤养法建立大鼠慢性应激抑郁模型)、癫痫组和EAD组,每组13只,后2组利用氯化锂-匹罗卡品建立癫痫模型,于造模后第14天对癫痫模型进行抑郁筛选,每只大鼠以抑郁各指标评分分别入癫痫组和EAD组。于造模成功后第4周分别用免疫荧光染色检测额前皮质BDNF及Trk B免疫阳性细胞表达;用Western blot检测额前皮质BDNF及Trk B蛋白定量表达。结果与癫痫组比较,EAD组额前皮质BDNF和Trk B阳性细胞数减少[(26. 08±1. 18)个/视野vs (32. 86±2. 28)个/视野,P <0. 05;(26. 48±4. 66)个/视野vs (35. 86±4. 37)个/视野,P <0. 05]。与正常组和癫痫组比较,EAD组额前皮质BDNF和Trk B蛋白表达明显减少(0. 522±0. 028 vs 0. 912±0. 035和1. 248±0. 066,P <0. 05;0. 494±0. 015 vs 0. 663±0. 024和1. 035±0. 048,P <0. 05)。结论 EAD大鼠额前皮质BDNF、Trk B蛋白的表达减少可能与EAD发病有关。Objective To study the expressions of brain-derived neurotrophic factor(BDNF)and its high-affinity tropomyosin receptor kinase B(TrkB)protien in prefrontal cortex of rats with epilepsy-associated depression(EAD).Methods Fifty-two rats were randomly divided into control group,depression group,epilepsy group,and EAD group(13 in each group).EAD rats were screened on day 14 after an epilepsy model was induced by lithium chloride-pilocarpine.Expressioons of BDNF and TrkB protien in prefrontal cortex of rats were detected by Weatern blot with immunofluorescence staining at week 4 after the epilepsy model was established.Results The number of BNDF and TrkB positive cells was smaller in EAD group than in epilepsy group(26.08±1.18/per visual field vs 32.86±2.28/per visual field,P<0.05;26.48±4.66/per visual field vs 35.86±4.37/per visual field,P<0.05).The expression levels of BDNF and TrkB protein were significantly lower in EAD group than in control group and epilepsy group(0.522±0.028 vs 0.912±0.035 and 1.248±0.066,P<0.05;0.494±0.015 vs 0.663±0.024 and 1.035±0.048,P<0.05).Conclusion Down-regulated expressions of BDNF and TrkB protein in prefrontal cortex of rats are related with the occurrence of EAD.

关 键 词：癫痫 脑源性神经营养因子 受体蛋白质酪氨酸激酶类 抑郁 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R749.4[医药卫生—临床医学]