2,4-表油菜素内酯对镉胁迫下大麦幼苗生长的影响  

Effect of 2,4-Brassinosteroid on Growth of Hordeum vulgare Seedlings under Cd-stress

作　　者：李晓科[1] 武玉珍[1] 张义贤[2] LI Xiao-ke;WU Yu-zhen;ZHANG Yi-xian(School of Biology Science and Technology,Jinzhong College,Jinzhong,Shanxi 030619,China;School of Life Science,Shanxi University,Taiyuan,Shanxi 030006,China)

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030619 [2]山西大学生命科学学院,山西太原030006

出　　处：《福建农业学报》2018年第12期1251-1256,共6页Fujian Journal of Agricultural Sciences

基　　金：山西省自然科学基金项目(201701D121083)

摘　　要：采用室内培养法,研究2,4-表油菜素内酯(2,4-EBL)对Cd胁迫下大麦幼苗的生长影响。结果表明,在所试Cd质量浓度(20mg·L^(-1))和2,4-EBL质量浓度(0.2mg·L^(-1))处理下,与对照相比,Cd处理使大麦幼苗生长受到抑制,叶片受到损伤,表现为根长、苗长、生物量、叶绿素含量、可溶性糖和可溶性蛋白含量、POD和CAT活性等指标下降,而叶片相对电导率、MDA、O_2^-含量、脯氨酸含量、SOD活性等指标增加。喷施2,4-EBL后,与单一Cd处理组相比,大麦幼苗根长、苗长、生物量和叶绿素含量显著增加,促进了光合作用和幼苗生长,降低了叶片相对电导率、MDA及O_2^-含量,而使渗透调节物质(可溶性糖、脯氨酸、可溶性蛋白)和抗氧化酶(SOD、POD、CAT)活性增加。表现出2,4-EBL对Cd胁迫后的大麦幼苗具有一定的缓解作用,增强了大麦在逆境中的适应能力。Effect of 2,4-brassinosteroid on the growth of barley(Hordeum vulgare)seedlings under Cd-stress were studied by an indoor culture experiment.In comparison with control,the treatment of Cd at 20 mg·L^-1 inhibited the seedling growth and damaged the leaf physiology manifesting in the decreased root length and plant height,reduced contents on biomass,chlorophyll,soluble sugars and soluble protein,declined activities of POD and CAT,increased relative electrical conductivity,heightened contents of MDA,O-2 and proline,and elevated activity of SOD.After spraying 2,4-brassinosteroid at 0.2 mg·L^-1,the Cd-stressed seedlings significantly increased on the root length,plant height,biomass and chlorophyll.The treatment promoted the photosynthesis and seedling growth,reduced the leaf relative conductivity,MDA and O-2,and facilitated an osmotic adjustment with the substances(e.g.,soluble sugars,proline and soluble protein),and enhanced the antioxidant enzymatic activities on SOD,POD and CAT.It appeared that the plant hormone could alleviate the ill-effects of Cd-stress on and enrich the adaptability in the battle against the adversity for the barley seedlings.

关 键 词：镉胁迫 油菜素内酯 大麦 幼苗生长 

分 类 号：X171.5[环境科学与工程—环境科学]