精神分裂症病人外周血差异表达miRNA-181b的生物信息学分析  

Bioinformatics analysis of the differential expression of miRNA-181b in peripheral blood of schizophrenic patients

作　　者：宋红涛[1] 王立金[1] 沐林林[1] 许华山[1] 谢杏利[1] 翟长平[2] 张俊[2] 吴延海[2] 崔明[2] SONG Hong-tao;WANG Li-jin;MU Lin-lin;XU Hua-shan;XIE Xing-li;ZHAI Chang-ping;ZHANG Jun;WU Yan-hai;CUI Ming(School of Psychiatric Medicine,Bengbu Medical College,Bengbu Anhui 233030;Anhui Veterants Hospital,Huaiyuan Anhui 233400,China)

机构地区：[1]蚌埠医学院精神医学院,安徽蚌埠233030 [2]安徽省荣军医院,安徽蚌埠233400

出　　处：《蚌埠医学院学报》2019年第2期173-177,共5页Journal of Bengbu Medical College

基　　金：蚌埠医学院自然科学基金重点项目(BYKY1616ZD)

摘　　要：目的:对精神分裂症病人外周血中差异表达的miRNA-181b进行靶基因、转录因子和信号通路的分析。方法:采用DIANA-TarBase数据库分析miRNA-181b靶基因,应用DIANA-miPath V.3软件对miRNA-181b的靶基因进行GO功能富集分析和KEGG信号通路富集分析,运用DIANA-miRGen v3.0数据库分析miRNA-181b的转录因子。结果:miRNA-181b靶基因共1 344个,其中显著表达的共有靶基因是ARHGAP11A、PPA1、YY1和ZC3H12B;miRNA-181b的靶基因广泛参与生物学过程的调节,包括分子功能、RNA结合、细胞组成等过程;KEGG生物信息学分析发现miRNA-181b的靶基因富集了12条信号通路,包括hippo信号通路、p53信号通路、HIF-1信号通路、赖氨酸的降解等;与miRNA-181b显著关联的转录因子有7个,其中JUN、EGR1、MEF2C与精神分裂症有显著关联。结论:对精神分裂症病人外周血中差异表达的miRNA-181b进行生物信息学分析,发现了多个靶基因、信号通路和转录因子与精神分裂症的发病有密切关联。Objective:To analyze the target gene,transcription factor and signal pathway of the differential expression of miRNA-181b in the peripheral blood of schizophrenic patients.Methods:The miRNA-181b target gene was analyzed using the DIANA-TarBase database,the GO function and KEGG pathway enrichment of miRNA-181b gene were analyzed using DIANA-miPath V.3 software,and the transcription factors of miRNA-181b were analyzed using the DIANA-miRGen v3.0 database.Results:There were 1 344 target genes of miRNA-181b,and the target genes ARHGAP11A,PPA1,YY1 and ZC3H12B significantly expressed.The target genes of miRNA-181b were widely involved in the regulation of biological processes(including molecular function,RNA binding and cell composition).The results of KEGG bioinformatics analysis showed that the target gene of miRNA-181b enriched 12 signal pathways,which included the hippo signal pathway,p53 signal pathway,HIF-1 signal pathway and lysine degradation.Seven transcription factors were significantly associated with miRNA-181b,and JUN,EGR1 and MEF2C were significantly associated with schizophrenia.Conclusions:The multiple target genes,signal pathways and transcription factors are closely related to the onset of schizophrenia using the bioinformatics analysis of the differential expression of miRNA-181b in the peripheral blood of schizophrenic patients.

关 键 词：精神分裂症 生物信息学 miRNA-181b 

分 类 号：R749.3[医药卫生—神经病学与精神病学]