Notch信号通路促进滑膜肉瘤细胞SW982增殖和侵袭的研究  

Notch Signaling Promotes Proliferation and Migration of SW982 Synovial Sarcoma Cells

作　　者：高天[1] 余铃[2] 李舒[1] 刘佳勇[1] 白楚杰[1] 薛瑞峰[1] 张路[1] 方志伟[1] 樊征夫[1] GAO Tian;YU Ling;LI Shu;LIU Jiayong;BAI Chujie;XUE Ruifeng;ZHANG Lu;FANG Zhiwei;FAN Zhengfu(Department of Bone and Soft Tissue Tumor,Key Laboratory of Carcinogenesis and Translational Research( Ministry of Education),Peking UniversitySchool of Oncology,Beijing Cancer Hospital & Institute,Beijing 10014,China;Department of Orthopedics,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]北京大学肿瘤医院暨北京市肿瘤防治研究所骨与软组织肿瘤科恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142 [2]武汉大学人民医院骨1科,武汉430060

出　　处：《中国医科大学学报》2019年第3期210-215,共6页Journal of China Medical University

基　　金：国家自然科学基金(81802689)

摘　　要：目的探讨Notch信号通路对人滑膜肉瘤增殖及侵袭潜能的影响。方法培养人滑膜肉瘤细胞SW982和人滑膜细胞,观察2种细胞中Notch信号通路相关蛋白的表达情况。利用NICD1过表达、CFB1干扰慢病毒及γ-分泌酶抑制剂DAPT调控SW982中Notch信号通路,通过CCK-8及划痕试验对Notch信号通路能否影响人滑膜肉瘤细胞SW982进行验证。结果相比于正常滑膜细胞,Notch信号通路活性在人滑膜肉瘤中显著增高(P <0.05)。上调Notch信号通路后SW982增殖及侵袭能力明显增高,而下调Notch信号通路会导致SW982增殖及侵袭能力明显降低(P <0.05)。结论抑制Notch信号通路可以显著降低人滑膜肉瘤的增殖和侵袭能力。Objective To investigate the effect of the Notch signaling pathway on the proliferation and invasion of human SW982 synovial sarcoma cells. Methods SW982 cells and normal human synovial cells were routinely cultured,and the expression of proteins related to the Notch pathway was compared. The Notch signaling pathway was manipulated by NICD1 overexpression,CFB1 shRNA lentivirus, and the γ-secretase inhibitor,DAPT. CCK-8 and wound healing assays were carried out to investigate the role of the Notch signaling pathway in SW982 cells. Results The Notch signaling pathway clearly showed higher activity in human SW982 synovial sarcoma cells than in normal human synovial cells( P < 0.05). The proliferation and invasion of SW982 cells were significantly upregulated by overexpressing NICD1;however,were suppressed by downregulating the Notch signaling pathway using CFB1 shRNA or DAPT( P < 0.05). Conclusion Our findings demonstrate that the proliferation and invasion of human SW982 synovial sarcoma cells are dependent on Notch signaling pathway activity .

关 键 词：滑膜肉瘤 NOTCH信号通路 增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]