miR-362-3p在喉癌组织中的表达及其对喉癌Hep-2细胞迁移的影响  

miR-362-3p Expression in Laryngeal Cancer Tissues and Its Effect on Migration of Hep-2 Cells

作　　者：赵越[1] 孙媛媛 佟雪 岳鹏杰 富伟能[1] ZHAO Yue;SUN Yuanyuan;TONG Xue;YUE Pengjie;FU Weineng(Department of Genetics,College of Basic Medical Science,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学基础医学院遗传学教研室,沈阳110122

出　　处：《中国医科大学学报》2019年第3期236-239,共4页Journal of China Medical University

基　　金：辽宁省重点研发计划(2017225021);辽宁省高等学校基本科研项目(LQNK201726)

摘　　要：目的探讨miR-362-3p在喉癌组织中的表达水平及其对喉癌Hep-2细胞迁移能力的影响。方法收集50例喉癌组织及对应癌旁组织标本,实时PCR方法检测miR-362-3p在喉癌组织和癌旁组织中表达水平,分析miR-362-3p的表达与喉癌患者临床病理特征的关系;将miR-362-3p模拟物、抑制剂以及阴性对照miRNA分别转染喉癌Hep-2细胞,实时PCR检测转染效率;划痕和Transwell实验检测miR-362-3p对喉癌Hep-2细胞迁移能力的影响。结果 miR-362-3p在喉癌组织中表达水平显著高于癌旁组织(P <0.05),且与喉癌患者淋巴结转移及临床分期有关;上调miR-362-3p表达水平能促进喉癌Hep-2细胞的迁移能力,下调miR-362-3p表达水平能抑制喉癌Hep-2细胞的迁移能力(均P <0.05)。结论 miR-362-3p在喉癌组织中表达上调,且能够促进喉癌细胞的迁移能力,提示其发挥潜在癌基因作用。Objective To explore miR-362-3p expression in laryngeal cancer tissues and its effect on migration of Hep2 cells. Methods miR-362-3p expression in 50 pairs of tumor and adjacent normal tissues was detected by real-time PCR after sample collection. The relationships between miR-362-3p expression and clinical pathological characteristics in patients with laryngeal cancer were analyzed. miR-362-3p mimic,inhibitor,and control microRNA were transfected into Hep-2 cells. Transfection efficiency was determined by real-time PCR . Wound-healing and transwell assays were used to evaluate Hep-2 migration. Results miR-362-3p expression was significantly higher in cancer tissues than in adjacent normal tissues( P < 0.05). miR-362-3p expression was statistically significantly related to node metastasis and clinical stage. Transfection with miR-362-3p mimic and inhibitor significantly increased and decreased Hep-2 cell migration, respectively( both P < 0.05). Conclusion miR-362-3p is up-regulated in laryngeal cancer tissues and promotes laryngeal cancer cell migration,suggesting that it acts as a potential oncogene in laryngeal cancer.

关 键 词：miR-362-3p表达 喉癌 迁移 

分 类 号：R739.6[医药卫生—肿瘤]