1-甲基色氨酸作用卵巢癌耐药细胞后对免疫细胞功能的影响  

Effects of 1-methyltryptophan on immunological function of drug-resistant ovarian cancer cells

作　　者：冯勤梅[1] 候茜媛 吕真娇 Feng Qinmei;Hou Qianyuan;Lyu Zhenjiao(Department of Obstetrics and Gynecology, Shanxi Province People′s Hospital,Taiyuan 030012,China)

机构地区：[1]山西省人民医院妇科,太原030012

出　　处：《中国药物与临床》2019年第6期863-866,共4页Chinese Remedies & Clinics

基　　金：山西省自然科学基金(201701D121156)

摘　　要：目的应用1-甲基色氨酸(MT-1)干预卵巢癌耐药细胞SKOV3/CBP,探讨其干预耐药癌细胞能否对免疫细胞增殖及功能有影响。方法将MT-1与卵巢癌耐药细胞株即SKOV3/CBP细胞株共培养后加入淋巴细胞,以未加入MT-1的SKOV3/CBP细胞株为对照,四甲基偶氮唑盐(MTT)法监测淋巴细胞的增殖能力,随后分别将耐药SKOV3/CBP及SKOV3/CBP+MT-1与自然杀伤细胞(NK)细胞、CD8+T细胞共培养,乳酸脱氢酶(LDH)检测NK细胞的杀伤能力,酶联免疫斑点检测(ELISPOT)CD8+T细胞的杀伤能力。结果与未加入MT-1组对比,加入MT-1组SKOV3/CBP细胞中的淋巴细胞的增值,NK细胞的杀伤能力及CD8+T细胞的杀伤能力增强且差异有统计学意义(P<0.05)。结论 MT-1可提高卵巢癌耐药细胞对免疫细胞作用的敏感性,故MT-1可作为卵巢癌耐药治疗的新手段。Objective To investigate whether 1-methyltryptophan (MT-1) has any effects on proliferation and immunological functions of drug-resistant ovarian cancer cells through study using MT-1 intervention of the drug-resistant SKOV3/CBP cell line. Methods Drug-resistant ovarian cancer SKOV3/CBP cell line was co-cultured with MT-1, and then added with lymphocytes. SKOV3/CBP cells without MT-1 treatment were used as control. MTT method was used to monitor the proliferation of lymphocytes. Subsequently, the drug-resistant SKOV3/CBP and SKOV3/CBP^+MT-1 were co-cultured with NK cells and CD8^+ T cells, respectively. LDH was used to monitor the killing ability of NK cells, and ELISPOT to monitor the killing ability of CD8^+ T cells. Results Compared with the group without MT-1, the proliferation, NK cell killing ability and CD8^+ T cell killing ability of MT-1 treated SKOV3/CBP cells were increased, with statistically significant difference (P<0.05). Conclusion MT-1 may increase the sensitivity of drug-resistant ovarian cancer cells to immune cells, and therefore can be used as a new treatment for drug-resistant ovarian cancers.

关 键 词：卵巢 抗药性 肿瘤 MT-1 免疫 细胞 

分 类 号：R737.31[医药卫生—肿瘤]