LC3在成釉细胞瘤中的表达及意义  

Expression and Significance of LC3 in Ameloblastomas

作　　者：黄碧莹 刘洁 钟鸣[1,3] 王珺婷 王小玢 康媛媛[1] 孙妍[1] 乔雪[3] HUANG Biying;LIU Jie;ZHONG Ming;WANG Junting;WANG Xiaobin;KANG Yuanyuan;SUN Yan;QIAO Xue(Department of Oral Pathology,Hospital of Stomatology,China Medical University,Shenyang 110002,China;The Science Experiment Center,China Medical University,Shenyang 110122,China;Department of Central Laboratory,Hospital of Stomatology,China Medical University,Shenyang110002,China)

机构地区：[1]中国医科大学附属口腔医院病理科,沈阳110002 [2]中国医科大学科学实验中心,沈阳110122 [3]中国医科大学附属口腔医院中心实验室,沈阳110002

出　　处：《中国医科大学学报》2019年第2期101-104,共4页Journal of China Medical University

基　　金：国家自然科学基金(81470758);辽宁省教育厅科学技术研究项目(LK201626);沈阳市中青年科技创新人才支持计划(RC170078);沈阳市科技计划(F16-102-4-00)

摘　　要：目的探讨自噬标记蛋白微管相关蛋白1轻链3 (LC3)在成釉细胞瘤组织中的表达及意义。方法应用免疫组织化学染色方法检测104例成釉细胞瘤组织及20例正常口腔黏膜组织中LC3的表达,通过半定量方法分析其表达与临床病理资料的关系。结果 LC3在成釉细胞瘤上皮细胞中的阳性表达率显著高于正常口腔黏膜组织,差异有统计学意义(P <0.05);LC3的表达在性别、年龄、是否复发方面的差异无统计学意义(P> 0.05);发生于下颌骨的成釉细胞瘤中LC3的阳性表达率高于发生于上颌骨及牙龈的成釉细胞瘤,差异有统计学意义(P <0.05);实性/多囊型中LC3的阳性表达率显著高于其他3种病理分型,差异有统计学意义(P <0.05)。结论成釉细胞瘤中自噬活性显著高于正常口腔黏膜组织,自噬过程在成釉细胞瘤的发生发展中发挥一定作用,可能对成釉细胞瘤的局部侵袭性有一定影响。Objective To investigate the expression and significance of microtubule-associated protein 1 light chain 3(LC3),an autophagy marker protein,in ameloblastomas.Methods Immunohistochemical methods were employed to evaluate the expression of LC3 in104 cases of ameloblastomas and 20 cases of normal oral mucosal tissues.The results were analyzed by a semiquantitative analysis method.Results The reactivity of LC3 in the epithelial cells of ameloblastomas was positive,and the positivity rate was significantly higher than that in normal oral mucosal tissues(P<0.05).There were no significant differences between the LC3 expression levels with respect to age,gender,or recurrence(P<0.05).The positivity rate in mandible ameloblastomas was significantly higher than that in maxilla and gingiva ameloblastomas(P<0.05).The reactivity for LC3 was significantly higher in solid ameloblastomas than that in the other three tissue types(P<0.05).Conclusion Autophagic activity in ameloblastomas was higher than that in normal oral mucosal tissues.This suggests that autophagy plays an important role during tumorigenesis,and contributes to the local invasion of ameloblastomas.

关 键 词：微管相关蛋白1轻链3 成釉细胞瘤 自噬 免疫组织化学 

分 类 号：R780.2[医药卫生—口腔医学]