Robo4敲除促进大鼠缺血心肌微血管生成  

Robo4 Knockout Improved Myocardial Neovascularization in Rats with Acute Myocardial Infarction

作　　者：周蓉芳[1] 毛建华[2] 寿晓玲[1] 王雁 王伯忠[1] ZHOU Rong-fang;MAO Jian-hua;SHOU Xiao-ling(Zhejiang Hospital, Zhejiang 310013, China)

机构地区：[1]浙江医院心脏康复科,310013 [2]浙江医院放射科,310013

出　　处：《心脑血管病防治》2019年第1期61-63,共3页CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT

基　　金：浙江省医药卫生一般研究计划(编号:2015KYA007)

摘　　要：目的探讨Robo4敲除对大鼠急性心肌梗死后缺血心肌微血管生成的影响。方法采用结扎左冠状动脉主干方法复制大鼠急性心肌梗死模型,分为Robo4敲除大鼠假手术组(Robo4 sham)、SD大鼠假手术组(SD sham)、Robo4敲除大鼠心肌梗死组(Robo4 MI)和SD大鼠心肌梗死组(SD MI)。14天后提取大鼠MI边缘区心肌组织,VWF8免疫组织化学染色,检测微血管生成密度(MVD)。结果两假手术组间MI边缘区MVD无明显变化[(2.27±0.53)个比(1.89±0.34)个,P>0.05]。与SD sham组相比,SD MI组MI边缘区MVD显著增加[(16.63±3.13)个比(1.89±0.34)个,P<0.01]。与SD MI组相比,Robo4 MI组MI边缘区MVD显著增加[(22.49±3.05)个比(16.63±3.13)个,P<0.05]。结论大鼠急性心肌梗死14天后缺血心肌微血管生成增加,且Robo4敲除可以促进缺血心肌微血管生成。Objective To investigate the effects of Robo4 knockout on rats’ myocardial neovascularization after acute myocardial infarction. Methods Use left main coronary artery ligation to copy acute myocardial infarction rats models, which were randomly divided into Robo4 sham group, SD sham group, Robo4 MI group and SD MI group. In 14 days after ligation of the left main coronary artery, cardiac muscle tissue were extracted from MI fringe area in rats, microvascular density(MVD) was measured by VWF8 immunohistochemistry. Results The MVD had no significantly difference between Robo4 sham group and SD sham group (2.27±0.53 vs 1.89±0.34, P >0.05). Compared with SD sham group, MVD was effectively increased in SD MI group (16.63±3.13 vs 1.89±0.34, P <0.001). Compared with SD MI group, MVD was significantly increased in Robo4 MI group (22.49±3.05 vs 16.63±3.13, P =0.017). Conclusions Myocardial neovascularization was appeared 14 days after acute myocardial infarction, and Robo4 knockout could improve it.

关 键 词：Robo4 急性心肌梗死 微血管生成 

分 类 号：R361[医药卫生—病理学] R542[医药卫生—基础医学]