胃癌组织中NEDD4L的表达及其临床意义  

Expression of NEDD4L in gastric carcinoma and its clinical significance

作　　者：蒋兴旺 闫强[1] 张尚鑫[1] 孙若川 李德关 鲁明典[1] 张震[1] 李永翔[1] Jiang Xingwang;Yan Qiang;Zhang Shangxin(Dept of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022

出　　处：《安徽医科大学学报》2019年第1期107-111,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金面上项目(编号:81672389);安徽省科技攻关重大计划项目(编号:1704a0802167);安徽高校自然科学研究项目(编号:KJ2016A329)

摘　　要：目的研究胃癌中神经前体细胞表达发育下调样基因4(NEDD4L)的表达及其临床意义。方法分别采用实时荧光定量PCR和蛋白质免疫印迹法检测25对胃癌组织及其对应癌旁正常胃组织中NEDD4L的mRNA和蛋白表达水平,采用免疫组化方法(IHC)检测NEDD4L在124例胃癌组织和25例癌旁正常胃组织中的表达情况,并统计分析其表达与胃癌患者临床病理参数及预后之间的关系。结果NEDD4L在胃癌组织中的mRNA和蛋白表达水平均显著低于对应癌旁正常组织(P <0. 05)。免疫组化结果显示NEDD4L在胃癌组织中的阳性表达率明显低于癌旁正常组织(P <0. 05)。NEDD4L的表达与肿瘤组织分化程度(P <0. 05)、浸润深度(P <0. 05)和临床TNM分期(P <0. 05)显著相关,且NEDD4L高表达的胃癌患者其预后较好(P <0. 001)。结论 NEDD4L在胃癌组织中低表达,可能与胃癌的发生发展密切相关,且可能作为判断胃癌预后的潜在指标。Objective To investigate the expression of neural precursor cell-expressed developmentally down-regulated 4 like( NEDD4L) and its clinical significance in gastric cancer. Methods The expression levels of NEDD4L mRNA and protein were detected in 25 pairs of gastric carcinoma tissues and their corresponding normal tissues by real-time fluorescence quantitative PCR and Western blot assay. Immunohistochemistry( IHC) was used to observethe expression of NEDD4L protein in 124 cases of gastric cancer tissues and 25 cases of normal tissues. Then the relationship of the expression of NEDD4L protein with the clinical pathological parameters and prognosis of gastric cancer patients was analyzed by statistical methods. Results The expression of NEDD4L mRNA and protein in gastric cancer tissues was significantly lower than that in normal tissues( P < 0. 05). In addition,the result of Immunohistochemistry revealed that the positive expression rate of NEDD4L protein in gastric cancer tissue was significantly lower than that in normal tissue( P < 0. 05). The expression of NEDD4L protein was significantly correlated with the degree of tumor tissue differentiation( P < 0. 05),depth( P < 0. 05) and clinical TNM staging( P <0. 05),and overexpression of NEDD4L protein had a notable correlation with favorable prognosis of gastric cancer patients( P < 0. 001). Conclusion The low expression of NEDD4L in gastric cancer tissues may be closely related to the occurrence and development of gastric cancer,and may be a potential indicator for judging the prognosis of gastric cancer.

关 键 词：胃癌 神经前体细胞表达发育下调样基因4 预后 

分 类 号：R735.2[医药卫生—肿瘤]