KIF20A在胃癌中的表达与预后的关系  

The relationship between KIF20A expression and prognosis in gastric cancer

作　　者：盛燚 张尚鑫[1] 闫强[1] 孙若川 李德关 鲁明典[1] 张震[1] 李永翔[1] Sheng Yi;Zhang Shangxin;Yan Qiang(Dept of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022

出　　处：《安徽医科大学学报》2019年第1期116-120,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81672389);安徽省科技攻关重大计划项目(编号:1704a0802167);安徽高校自然科学研究项目(编号:KJ2016A329)

摘　　要：目的研究驱动蛋白家族20A(KIF20A)在胃癌中的表达及其临床意义。方法收取37对新鲜胃癌组织及其配对正常组织,实时荧光定量PCR(qRT-PCR)法检测组织中KIF20A的mRNA表达水平。此外,构建由122例胃癌组织和24例随机正常胃组织构成的组织微阵列,免疫组化法检测KIF20A的表达,评价KIF20A阳性表达与胃癌临床病理参数和预后的关系。siRNA沉默胃癌细胞系中KIF20A的表达,Western blot法检测细胞系中KIF20A的表达变化。CellTiter-Glo法和克隆形成实验检测细胞增殖能力的变化。结果KIF20A mRNA表达水平在胃癌组织中显著高于其配对正常组织(P <0. 05)。与此一致,组织微阵列中胃癌KIF20A的表达显著高于正常对照(P <0. 05)。KIF20A阳性表达与胃癌组织分化差相关(P=0. 036),生存分析显示KIF20A阳性表达与胃癌患者预后差相关[阳性组总生存期(40. 16±24. 65)月,阴性组总生存期(48. 91±21. 84)月](P=0. 022)。体外试验显示下调KIF20A表达能够抑制AGS细胞的增殖能力。结论胃癌组织中KIF20A在转录和翻译水平均过表达,下调KIF20A的表达能够抑制胃癌细胞增殖。KIF20A的阳性表达是影响胃癌临床预后的风险因素。Objective To detect the expression of kinesin family member 20A( KIF20A) in gastric cancer and adjacent normal mucosal tissue,and to explore the relationship between KIF20A expression and clinicopathological characteristics in gastric cancer( GC). Methods mRNA level of KIF20A were detected by quantitative real-time PCR( qRT-PCR) in 37 pairs of fresh GC and corresponding normal specimens. Immunohistochemistry assay based on a tissue microarray( TMA) consisting of 146 cases was performed to evaluate the expression,clinicopathologic characteristics and prognosis value of KIF20A. After silencing KIF20A for GC cell lines,cell viability and colony formation assay were carried out to evaluate the effects of KIF20A on GC in vitro. Results KIF20A expression level was increased in GC( P < 0. 05). TMA showed that percentage of KIF20A(+) is significantly higher in GC tissues( P < 0. 05). Pearson χ~2 test method indicated the close relationship between KIF20A and histological grade( P =0. 036). Survival analysis showed that median survival for patients with KIF20A was( 40. 16 ± 24. 65) months,which was lower than patients without KIF20A( 48. 91 ± 21. 84) months( P = 0. 022). Besides,silencing KIF20A markedly inhibited cell viability of AGS in vitro. Conclusion KIF20A expression is upregulated at both the transcriptional and translational levels in GC. Overall survival is lower in GC patients with KIF20A. Down-regulation of KIF20A can inhibit cell proliferation in vitro. KIF20A is an independent prognostic factor for GC.

关 键 词：KIF20A 胃癌 预后 增殖 

分 类 号：R735.2[医药卫生—肿瘤]