炎症性肠病患者结肠组织和血清中白细胞介素34的表达  

Expression of interleukin-34 in patients with inflammatory bowel disease

作　　者：王许平 王素敏[1] 孙亚星 耿丽 王丹丹[1] 杨贝贝 杜晓博 高闯 冯百岁[1] WANG Xuping;WANG Sumin;SUN Yaxing;GENG Li;WANG Dandan;YANG Beibei;DU Xiaobo;GAO Chuang;FENG Baisui(Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China)

机构地区：[1]郑州大学第二附属医院消化内科,河南郑州450014

出　　处：《胃肠病学和肝病学杂志》2019年第3期287-290,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金资助项目(81070288;81270452);河南省卫生计生科技创新型人才特聘学科带头人(豫卫科[2016]32号);河南省自然科学基金项目(162300410268);河南省医学科技攻关计划项目(201502015)

摘　　要：目的检测炎症性肠病(inflammatory bowel disease,IBD)患者结肠组织和血清中白细胞介素34(interleukin-34, IL-34)的表达,并进一步探讨IL-34与患者临床指标及疾病严重程度的关系,进而了解其在IBD中的意义。方法选取UC患者48例,其中缓解期9例,轻度11例,中-重度28例;CD患者21例,其中缓解期6例,轻度6例,中-重度9例。29名健康志愿者的血清作为对照(正常对照组),24例结肠癌手术切除的癌旁组织作为正常组织对照(癌旁组织对照组)。应用免疫组织化学法检测结肠组织中IL-34的表达。应用ELISA方法检测血清中IL-34的表达。并收集IBD患者临床指标血沉(erythrocyte sedimentation rate, ESR)及C反应蛋白(C-reactive protein, CRP)数据。结果活动期患者IL-34水平在结肠组织和血清中的表达差异均有统计学意义(P<0.05),UC活动期及CD活动期患者均高于对照组和缓解期(P<0.05),对照组与缓解期比较,差异无统计学意义(P>0.05)。UC组及CD组血清IL-34水平中-重度组分别高于轻度组(P<0.05)。血清IL-34水平与疾病活动度、ESR及CRP呈正相关。结论 IBD患者结肠组织及血清中IL-34明显升高,提示IL-34在IBD的发生、发展过程中可能有重要意义。Objective To detect the expression of interleukin-34 (IL-34) in patients with inflammatory bowel disease (IBD) and to investigate the correlation between serum IL-34 and other estabilished serum markers and disease severity. Methods A total of 48 patients with ulcerative colitis (UC) were chosen, including 28 patients with moderate to severe UC, 11 patients with mild UC, 9 patients in remission, and 21 patients with Crohn’s disease (CD), including 9 patients with moderate to severe CD, 6 patients with mild CD, 6 patients in remission. Blood samples from 29 healthy volunteers were served as serum controls. The colonic tissues from 24 patients with colon cancer were served as tissue controls. Immunohistochemistry was used to detect the expression of IL-34 in colonic tissue. ELISA was used to detect the expression of IL-34 in serum. The data of erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were collected from patients with IBD. Results The expression of IL-34 in colonic mucosa and serum in active UC group and active CD group was significantly different from control group and remission group respectively ( P <0.05). There was no significant difference between the remission group and the control group ( P >0.05). The serum levels of IL-34 in moderate to severe UC group and mild UC group were significantly different, while the same to CD group ( P <0.05). What’s more, the levels of serum IL-34 were positively correlated with disease activity, ESR and CRP. Conclusion IL-34 is significantly increased in colonic tissue and serum of patients with IBD, suggesting that IL-34 may be important in the genesis and development of IBD.

关 键 词：白细胞介素34 炎症性肠病 C反应蛋白 血沉 

分 类 号：R574.62[医药卫生—消化系统]