丹参水提液对碘乙酸钠所致大鼠骨关节软骨退变的预防作用  

Preventive effect of water extract of Danshen on degeneration of osteoarticular cartilage induced by monosodium iodocaetate in rats

作　　者：闵亚林 姜思羽 吴迪生 陈文双 许碧莲 MIN Ya-lin;JIANG Si-yu;WU Di-sheng;CHEN Wen-shuang;XU Bi-lian(Dept of Pharmacology,Guangdong Medical University,Zhanjiang Guangdong 524023,China;Guangdong Key Lab for Research and Developmentof Natural Drugs,Guangdong Medical University,Zhanjiang Guangdong 524023,China)

机构地区：[1]广东医科大学药理学教研室,广东湛江524023 [2]广东医科大学广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中国药理学通报》2019年第4期478-483,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81373499)

摘　　要：目的探讨丹参水提液对碘乙酸钠所致大鼠骨关节软骨退变的预防作用。方法♀SD大鼠27只随机分为假手术组、碘乙酸钠组、丹参水提液组。假手术组左膝关节腔内注射生理盐水,碘乙酸钠和丹参水提液组注射碘乙酸钠(60 g·L^(-1))。注射碘乙酸钠后d 2开始灌胃给药,假手术组和碘乙酸钠组给予生理盐水(5 mL·kg^(-1)),丹参水提液组给予丹参水提液(5 mL·kg^(-1)),每天给药1次,连续6周。实验结束后处死大鼠,取血清检测MMP-13、IL-6、TNF-α;取股骨进行番红O-固绿染色;取胫骨进行Micro-CT检测。结果与假手术组相比,碘乙酸钠组血清MMP-13和股骨内侧关节软骨病理学评分升高(P<0.01),胫骨内侧关节的软骨厚度和软骨体积减低(P<0.01)。与碘乙酸钠组相比,丹参水提液组血清MMP-13和股骨内侧关节软骨病理学评分降低(P<0.05,P<0.01),胫骨内侧关节的软骨厚度和软骨体积增加(P<0.01,P<0.05)。结论丹参水提液对碘乙酸钠所致大鼠骨关节软骨退变有预防作用。Aim To explore the preventive effect of water extract of Danshen(DWE) on degeneration of osteoarticular cartilage induced by monosodium iodocaetate(MIA).Methods Twenty-seven female SD rats were randomly divided into sham group,MIA group and DWE group.Rats in sham group were injected a single intra-articular of saline in left knee,while rats in MIA and DWE group were injected a single intra-articular of MIA(60 g·L^-1 ).The rats were administered on the second day after the injection of MIA.The rats in sham group and MIA group were given 5 mL·kg^-1 of saline,and the rats in DWE group received 5 mL·kg^-1 of DWE every day for six weeks.At the end of the experiment,the serum was tested for MMP-13,IL-6 and TNF-α by enzyme-linked immunosorbent assay(ELISA).The left knee femur of rats was taken for safranine O-fixed green staining and tibia was collected for Micro-CT assay.Results Compared with sham group,the serum level of MMP-13 and the pathological score of the medial femoral articular cartilage in MIA group significantly increased( P <0.01),while cartilage thickness and cartilage volume of the medial tibial articular cartilage markedly decreased( P <0.01).Compared with MIA group,the serum level of MMP-13 and the pathological score of the medial femoral articular cartilage in DWE group significantly decreased( P <0.05,P <0.01),while cartilage thickness and cartilage volume of the medial tibial articular cartilage increased( P <0.01,P <0.05).Conclusion DWE has a protective effect on degeneration of osteoarticular cartilage induced by monosodium iodocaetate.

关 键 词：骨性关节炎 碘乙酸钠 丹参水提液 MICRO-CT 大鼠 软骨 

分 类 号：R-332[医药卫生] R284.1