电离辐照损伤对小鼠睾丸组织氧化磷酸化信号通路的影响  

Effects of ionizing radiation injury on oxidative phosphorylation signaling pathway in testicular tissues of mice

作　　者：罗芳芳 李敏[2] 易辉燕 吴莎莎 唐莉[2] 王继生 LUO Fang-fang;LI Min;YI Hui-yan;WU Sha-sha;TANG Li;WANG Ji-sheng(College of Pharmacy,Southwest Medical University,Luzhou Sichuan 646000,China;the Third People’s Hospital of Mianyang · Sichuan Mental Health Center,Mianyang Sichuan 621000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]绵阳市第三人民医院(四川省精神卫生中心),四川绵阳621000

出　　处：《中国药理学通报》2019年第4期483-488,共6页Chinese Pharmacological Bulletin

基　　金：四川省应用基础研究计划项目(No 2014JY0058)

摘　　要：目的比较电离辐照损伤小鼠与正常小鼠的睾丸组织差异表达蛋白质,分析所得差异蛋白涉及的信号通路,以期从蛋白质组学角度阐述电离辐照对睾丸组织损伤的作用机制。方法采用^(60)Coγ射线辐照建立小鼠电离辐照损伤模型。运用比较蛋白质组学方法、iTRAQ联合LC-MS/MS检测技术,提取出小鼠辐照组与正常组睾丸组织比较后的差异表达蛋白质,进一步利用David6.8、String10.5和Cytoscape3.6.1数据库,对差异蛋白进行KEGG富集分析和相互作用分析。结果 KEGG富集分析鉴定出21条生物信号通路(P<0.05),其中富集于氧化磷酸化信号通路的差异蛋白有13个,均为表达下调。该通路涉及ATP合成酶、细胞色素、NADH脱氢酶这3类蛋白的多个亚基,它们主要参与线粒体电子传递、线粒体呼吸链复合物I装配、ATP生物合成等过程。结论电离辐照引起小鼠睾丸组织氧化磷酸化信号通路所涉及的13个差异蛋白均出现低表达,导致细胞机体ATP的合成障碍是辐射损伤的重要机制。Aim To compare the differential expression of proteins in testicular tissues of ionized irradiated mice and normal mice,in order to explain the mechanism of ionizing irradiation on testicular tissue damage from proteomics perspective.Methods The mouse ionizing radiation damage model was established by cobalt 60-ray irradiation.Comparative proteomics method,iTRAQ combined with LC-MS/MS detection technology were used to extract differentially expressed proteins of testis tissues from mouse irradiated group and normal group,then David6.8,String10.5 and Cytoscape 3.6.1 database were employed for KEGG enrichment analysis and interaction analysis on differential proteins.Results Twenty-one biological signaling pathways( P <0.05) were identified by KEGG enrichment analysis,with 13 differentially expressed proteins enriched in phosphoric oxide signaling pathways,and both were downgrades of expression.The pathway involved several subgroups of ATP synthase,cytochrome and NADH dehydrogenase,which were mainly involved in mitochondrial electron transfer,mitochondrial respiratory chain complex I assembly,ATP biosynthesis and so on.Conclusions Ionizing radiation is responsible for the expression of oxidative phosphorylation signaling pathway in mouse testis tissues.The low expression of 13 differential proteins leads to the synthesis of cell ATP.It is an important mechanism of radiation damage.

关 键 词：电离辐照 睾丸 蛋白质组学 ITRAQ KEGG 氧化磷酸化 

分 类 号：R-332[医药卫生] R122.4