三叉神经痛大鼠三叉神经节中BDNF的表达变化  

The expression changes of BDNF in trigeminal ganglia of rats with trigeminal neuralgia

作　　者：刘丽娟 尹灿灿 关书 葛会香 王聪睿 高云[2] 熊伟[1,4] LIU Li-juan;YIN Can-can;GUAN Shu;GE Hui-xiang;WANG Cong-rui;GAO Yun;XIONG Wei(Nanchang 330019,China;Basic Medical College of Nanchang University,Nanchang 330006,China;Second School of Clinical Medicine of Nanchang University,Nanchang 330019,China;the Key Lab of Oral Biomedicine of Jiangxi Province,Nanchang 330019,China)

机构地区：[1]南昌大学附属口腔医院预防科,江西南昌330019 [2]南昌大学基础医学院生理学教研室,江西南昌330006 [3]南昌大学第二临床医学院,江西南昌330019 [4]江西省口腔生物学重点实验室,江西南昌330019

出　　处：《中国药理学通报》2019年第4期576-580,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81460106;81760152)

摘　　要：目的观察脑源性神经生长因子(brain-derived neurotropic factor,BDNF)及其高亲和力受体TrkB在三叉神经痛(trigeminal neuralgia,TN)大鼠三叉神经节(trigeminal ganglion, TG)中的表达变化。方法 TN大鼠模型由眶下神经慢性损伤压迫模型(ION-CCI)制备。♂SD大鼠随机分为假手术组(Sham组)和TN模型组。测定两组大鼠损伤侧机械阈值,应用实时荧光定量PCR、免疫组织化学技术和免疫荧光双标技术,检测损伤侧TG中BDNF及TrkB表达变化,并测定损伤侧TG中前炎症因子TNF-α、IL-1β的变化。结果建模2周后,与假手术组相比,TN组的机械痛阈明显下调,TG中BDNF及TrkB较假手术组明显升高,同时前炎症因子TNF-α、IL-1β水平也明显升高(P<0.05)。结论 TN状态下,ION-CCI大鼠TG中BDNF及TrkB表达升高,可能参与TN的发病机制,促进了TN的痛觉传递。Aim To observe the expression changes of brain-derived neurotropic factor (BDNF) and receptor TrkB in trigeminal ganglion (TG) of trigeminal neuralgia (TN) rats.Methods Animal model of TN was established using the infraorbital nerve chronic injury compression model (ION-CCI).The sham operation group (sham group) and the TN model group were composed of randomly divided 180-220 g SD male rats.The mechanical threshold of the injury side of the two groups was determined.Quantitative real-time PCR(qPCR),immunohistochemistry and immunfluorescence methods were used to detect the expression of BDNF,TrkB,pro-inflammatory TNF-α and IL-1β in the injured TGs.Results After two weeks of modeling,the mechanical pain threshold was significantly down-regulated in TN group compared to sham group ( P <0.05).The expression of BDNF and TrkB in TGs of TN group was significantly higher than that in sham operation group.Besides,the levels of pro-inflammatory factors TNF-α and IL-1β also significantly increased in TGs of TN group compared with sham group ( P <0.05).Conclusions The expression of BDNF and TrkB in TG of ION-CCI rats increases in trigeminal neuralgia group,which may be involved in the pathogenesis of trigeminal neuralgia and promote the pain transmission of trigeminal neuralgia.

关 键 词：脑源性神经生长因子 TRKB 三叉神经节 三叉神经痛 TNF-α IL-1β 

分 类 号：R-332[医药卫生] R322.85