血浆间甲氧肾上腺素类放免分析试剂盒的初步鉴定  

The Preliminary Evaluation of RIA Kit of Plasma MNs

作　　者：贺嵩 张庚宽 周棱 曾玲 朱智慧 祝蓉 叶茂 李欣 兰竹 黄定德 HE Song;ZHANG Geng-kuan;ZHOU Ling;ZENG Ling;ZHU Zhi-hui;ZHU Rong;YE Mao;LI Xin;LAN Zhu;HUANG Ding-de(Department of Nuclear Medicine,First Affiliated Hospital of Army Medical University,Chongqing,China;Beijing North Institute of Biotechnology Co.,Ltd,Marketing,Beijing 100076,China)

机构地区：[1]陆军军医大学第一附属医院核医学科,重庆400038 [2]北京北方生物技术研究所有限公司,北京100076

出　　处：《标记免疫分析与临床》2019年第3期529-532,共4页Labeled Immunoassays and Clinical Medicine

基　　金：重庆市社会民生科技创新专项(编号:cstc2015shmszx120004)

摘　　要：目的对自行研制的甲氧肾上腺类放射免疫分析试剂盒的性能进行初步鉴定。方法分离49位门诊患者的血浆,应用自行研制的RIA试剂盒及LC-MS/MS法分别检测血浆中甲氧基肾上腺素(MN)和甲氧基去甲肾上腺素(NMN)的含量,探讨两种方法的相关性和一致性,并分析RIA试剂盒的精密度、回收率及灵敏度。结果两种检测方法得到的MN及NMN结果均明显正相关,相关系数r^2分别为0.97、0. 98,一致性分别为100%及86%;MNs放免试剂盒的批内及批间差异均小于15%,MN的回收率为89%～103%,NMN的回收率为97%～119%。MN的检出限为4.3 pg/mL,NMN的检出限为19. 8 pg/mL。结论自行研制的血浆MNs放射免疫分析试剂盒检测具有较高的精密度和准确度,与LC-MS/MS间具有较高的相关性与一致性,有望代替LC-MS/MS作为PPGL定性诊断的常规方法 。Objective The performance of the self-developed radioimmunoassay kit for MNs was preliminarily evaluated.Methods The plasma samples of 49 outpatients were obtained.The contents of methoxy-adrenaline(MN)and methoxy-noradrenaline(NMN)in plasma were detected by RIA kit and LC-MS/MS,respectively.The correlation and consistency between the two methods were investigated,and the precision,recovery and sensitivity of RIA kit were analyzed.Results The correlation coefficients r2 were 0.97 and 0.98,and the consistencies were 100%and 86%,respectively.The intra-and inter-assay differences of MNS RIA kits were less than 15%,the recovery of MN was 89%-103%,and the recovery of NMN was 97%-119%.The detection limit of MN was 4.3 pg/mL,and the detection limit of NMN was 19.8 pg/mL.Conclusion The self-developed plasma MNS radioimmunoassay kit has high precision and accuracy,and has good correlation and consistency with LC-MS/MS.It is expected to replace LC-MS/MS as a routine method for qualitative diagnosis of PPGL.

关 键 词：甲氧肾上腺素类 嗜铬细胞瘤 放射免疫法 高效液相色谱串联质谱法 

分 类 号：R736.6[医药卫生—肿瘤]