聚己内酯/Ⅰ型胶原/氟磷灰石复合支架的制备及生物相容性研究  

Preparation and biocompatibility of polycaprolactone/type Ⅰ collagen/fluoroapatite composite scaffold

作　　者：范瑞瑞 李欣聪 刘玉[1] 孙卫斌[1] 郭婷 FAN Ruirui;LI Xincong;LIU Yu;SUN Weibin;GUO Ting(Department of Periodontology,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China;Department of Endodontics,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院.南京市口腔医院牙周病科,江苏南京210008 [2]南京大学医学院附属口腔医院.南京市口腔医院牙体牙髓病科,江苏南京210008

出　　处：《口腔生物医学》2019年第1期12-16,共5页Oral Biomedicine

基　　金：国家自然科学基金(51772144;81500872);江苏省自然科学基金(BK20161389)

摘　　要：目的:制备聚己内酯(PCL)/Ⅰ型胶原(COLI)/氟磷灰石(FA)复合支架用于牙周组织再生,并进行生物相容性及成骨诱导性能的检测。方法:制备PCL/COLI静电纺丝纳米纤维膜,并在其表面合成FA矿化涂层,通过扫描电镜观察其形貌。同时分离培养人牙周膜细胞,并与支架浸提液共培养,通过CCK-8法检测细胞增殖情况,通过碱性磷酸酶(ALP)染色、茜素红染色和Von Kossa染色观察复合支架对人牙周膜细胞成骨分化的影响。结果:PCL/COLI/FA复合支架呈三维网状结构,FA晶体分布于支架表面。CCK-8结果证明人牙周膜细胞在复合支架上增殖情况良好。ALP染色、茜素红染色和Von Kossa染色结果提示复合支架可诱导人牙周膜细胞成骨分化。结论:PCL/COLI/FA复合支架生物相容性良好并具有成骨诱导潜能,有望作为牙周组织工程支架材料。Objective:To prepared Polycaprolactone/type I collagen/fluoroapatite(PCL/COLI/FA)composite scaffold for periodontal tissue regeneration,and to test its biocompatibility and osteogenic induction.Methods:PCL/COLI electrospun nanofiber membrane was prepared,and the fluoroapatite mineralized coating was synthesized on the surface.The morphology was observed by scanning electron microscopy.At the same time,human periodontal ligament cells were isolated and co-cultured with the scaffolds extract,and the cell proliferation was detected by CCK-8 method.The effects of composite scaffold on osteogenic differentiation of human periodontal ligament cells were observed by ALP staining,alizarin red staining and Von Kossa staining.Results:The PCL/COLI/FA composite scaffold showed a three-dimensional network structure,and the fluoroapatite crystals were distributed on the surface of the scaffold.The CCK-8 results showed that the human periodontal ligament cells proliferated well on the composite scaffold.ALP staining,alizarin red staining and Von Kossa staining results suggest that the composite scaffold can induce osteogenic differentiation of human periodontal ligament cells.Conclusions:The PCL/COLI/FA composite scaffold has good biocompatibility and osteogenic induction potential,and it is expected to be used as a scaffold material for periodontal tissue engineering.

关 键 词：聚己内酯 Ⅰ型胶原 氟磷灰石 人牙周膜细胞 成骨分化 

分 类 号：R780.2[医药卫生—口腔医学]