干扰PRMT2基因对肝癌细胞系生物学行为的影响  

Interference with PRMT2 gene on biological behavior of hepatoma cell lines

作　　者：明志勇 鞠佳霓 代全楷 黄山[1] 李科志[1] 邬国斌[1] 陈闯[1] 何剑波[1] 赵荫农[1] Ming Zhiyong;Ju Jiani;Dai Quankai;Huang Shan;Li Kezhi;Wu Guobin;Chen Chuang;He Jianbo;Zhao Yinnong(Affiliated Tumor Hospital of Guangxi Medical University,Guangxi Nanning 530021,China;Graduate School,Guangxi Medical University,Guangxi Nanning 530021,China)

机构地区：[1]广西医科大学附属肿瘤医院,广西南宁530021 [2]广西医科大学研究生院,广西南宁530021

出　　处：《现代肿瘤医学》2019年第7期1104-1109,共6页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81460426)

摘　　要：目的:研究抑制蛋白质精氨酸甲基转移酶2(protein arginine methyltransferase 2,PRMT2)基因的表达对肝癌细胞系生物学行为的影响。方法:qRT-PCR检测人肝癌细胞系(HepG2和SMMC-7721)和人正常肝细胞(LO2)中PRMT2的表达情况;设计并合成针对PRMT2基因的3对小干扰RNA,体外通过脂质体Lipofectamine 3000转染到相对高表达PRMT2的人肝癌细胞系HepG2和SMMC-7721中抑制PRMT2基因的表达,并设置阴性对照组(NC组)和空白对照组。qRT-PCR、Western blot检测转染后细胞PRMT2 mRNA水平和蛋白水平的变化。CCK-8法检测转染后细胞增殖能力,Transwell小室检测细胞体外迁移及侵袭能力。结果:与人正常肝细胞LO2相比,PRMT2基因在人肝癌细胞系HepG2和SMMC-7721中相对高表达。将siPRMT2转染HepG2和SMMC-7721细胞后,与NC组相比,siPRMT2-1和siPRMT2-2组的PRMT2 mRNA和蛋白表达均明显降低(P均<0.05),NC组与空白对照组无明显差异。与NC组相比,将siPRMT2-1和siPRMT2-2转染进HepG2和SMMC-7721细胞后,细胞增殖速度减慢、迁移和侵袭能力显著减弱(P均<0.05),NC组与空白对照组无明显差异。结论:RNA干扰抑制PRMT2表达后,抑制肝癌细胞的增殖、迁移和侵袭,PRMT2可能影响肝癌的预后。Objective:To investigate the effect of inhibition of PRMT2 gene expression on the biological behavior of hepatoma cell line.Methods:qRT-PCR was used to measure the expression of PRMT2 in human hepatoma cell lines(HepG2 and SMMC-7721) and normal hepatocytes(LO2).Three pairs of small interfering RNAs targeting PRMT2 gene were designed and synthesized,the expression of PRMT2 gene was inhibited by lipofectamine Lipofectamine 3000 transfected into human hepatoma cell lines HepG2 and SMMC-7721 with relatively high expression of PRMT2,a negative control group(NC group),blank control group were set up.The changes of PRMT2 mRNA and protein levels in transfected cells were detected by qRT-PCR and Western blot.The cell proliferation ability after transfection was detected by CCK-8 method,the migration invasive ability of cells was detected by Transwell chamber.Results:Compared with normal human hepatocytes(LO2),the PRMT2 gene is relatively highly expressed in the human hepatoma cell lines HepG2 and SMMC-7721.After transfection of siPRMT2 with HepG2 and SMMC-7721 cells,the expressions of PRMT2 mRNA and protein in siPRMT2-1 and siPRMT2-2 groups were significantly lower than those in NC group(P<0.05).There was no significant difference between NC group and blank control group.Compared with the NC group,the proliferation rate,migration,invasion ability of siPRMT2-1 and siPRMT2-2 transfected into HepG2 and SMMC-7721 were significantly decreased(P<0.05),there was no significant difference between NC group and blank control group.Conclusion:RNA interference inhibits the expression of PRMT2 and inhibits the proliferation,migration and invasion of liver cancer cells.PRMT2 may affect the prognosis of liver cancer.

关 键 词：肝癌 PRMT2 增殖 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]