米非司酮对异位子宫内膜腺上皮细胞中凋亡基因、侵袭基因表达的影响  

The effects of mifepristone on apoptosis gene and invasion gene expression in endometrial glandular epithelial cells

作　　者：姚霁航[1] 张建宇 李琳[1] 王涵 杨紫伟[2] 郭科军[1] Yao Jihang;Zhang Jianyu;Li Lin;Wang Han;Yang Ziwei;Guo Kejun(Department of Gynecology,the First Hospital of China Medical University,Liaoning Shenyang 110000,China;Department of Clinical Laboratory,the First Hospital of China Medical University,Liaoning Shenyang 110000,China)

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110000 [2]中国医科大学附属第一医院检验科,辽宁沈阳110000

出　　处：《现代肿瘤医学》2019年第7期1196-1199,共4页Journal of Modern Oncology

摘　　要：目的:探讨米非司酮对异位子宫内膜腺上皮细胞中多种凋亡基因、侵袭基因表达的影响。方法:本研究为双盲随机对照研究,选取78例子宫内膜异位症患者作为研究对象,随机分为米非司酮组与对照组,各39例。米非司酮组采用米非司酮+手术治疗,对照组仅行手术治疗,术后采用酶联免疫法检测两组侵袭基因、凋亡基因的蛋白表达量。结果:米非司酮组细胞凋亡率高于对照组,差异有统计学意义(P<0.05);异位子宫内膜腺上皮细胞中β-catenin、GSK3β、uPA、NF-κBp65、OPN等侵袭基因的蛋白表达量均明显低于对照组,差异有统计学意义(P<0.05);PTEN、Smac、Bax、Fas等促凋亡基因的蛋白表达量均高于对照组,差异有统计学意义(P<0.05);Ki-67、c-IAP1、Bcl-2、Livin、Id-1等凋亡抑制基因的蛋白表达量均明显低于对照组,差异有统计学意义(P<0.05)。结论:米非司酮能够影响异位子宫内膜腺上皮细胞中多种侵袭、凋亡基因的表达,可下调侵袭基因的表达,抑制侵袭;上调促凋亡基因的表达以及下调凋亡抑制基因的表达,促进细胞凋亡。Objective:To study the effects of mifepristone on various apoptosis genes and invasion genes expression in endometrial glandular epithelial cells.Methods:In this study,a double-blind randomized controlled study was conducted,and 78 cases of patients with endometriosis were selected as the study subjects.Mifepristone group was treated with mifepristone + surgery,and the control group was treated only with surgery.After surgery,the enzyme linked immunoassay was used to detect the protein expression of two groups of invasive genes and apoptosis genes.Results:The apoptosis rate of mifepristone group was higher than that in the control group,and the difference was statistically significant (P<0.05).The protein expression levels of β-catenin,GSK3β,uPA,NF-κBp65 and OPN were significantly lower than those in the control group,and the differences were statistically significant (P<0.05).PTEN,Smac,Bax,Fas and other apoptotic genes had higher protein expression levels than the control group,and the difference was statistically significant (P<0.05).The protein expression levels of Ki-67,c-IAP1,Bcl-2,Livin and Id-1 were significantly lower than those of the control group,and the difference was statistically significant (P<0.05).Conclusion:Mifepristone can affect the expression of multiple invasive and apoptotic genes in endometrial glandular epithelial cells,and can down-regulate the expression of invasive genes and inhibit invasion,up-regulate proapoptotic gene expression and down-regulate apoptosis suppressor gene expression,promote apoptosis.

关 键 词：米非司酮 子宫内膜异位症 腺上皮细胞 凋亡基因 侵袭基因 

分 类 号：R737.33[医药卫生—肿瘤]