葡萄糖与酵母膏配比对猴头菌胞外酶活性的影响  

Effects of the Ratio of Glucose and Yeast Extract on Extracellular Enzyme Activity of Hericium erinaceus

作　　者：张亚洁 龚教龙 于晶 赵志莹 阮航 范博文 李佐同 赵长江 ZHANG Ya-jie;GONG Jiao-long;YU Jing;ZHAO Zhi-ying;RUAN Hang;FAN Bo-wen;LI Zuo-tong;ZHAO Chang-jiang(College of Agriculture,Heilongjiang Bayi Agricultural University,Heilongjiang Engineering Technology Research Center for Crop Straw Utilization,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院/黑龙江省秸秆资源化利用工程技术研究中心,黑龙江大庆163319

出　　处：《黑龙江农业科学》2019年第3期133-137,共5页Heilongjiang Agricultural Sciences

基　　金：中央引导地方科技发展专项(ZY16A06);黑龙江八一农垦大学大学生创新创业计划训练(XC2016003)

摘　　要：为揭示碳源和氮源配比对猴头菌胞外酶活性的影响,选用葡萄糖作为碳源,酵母膏作为氮源,以猴头菌(Hericium erinaceus)菌种RT22为材料,基础培养基中葡萄糖与酵母膏配比为3∶1为对照,设置基础培养基中葡萄糖和酵母膏配制比例为5∶3、2∶1、7∶3、5∶2、7∶2、5∶1、6∶1、7∶1的液体培养基,摇床培养8 d后,取猴头菌菌液测定羧甲基纤维素酶、半纤维素酶、淀粉酶、滤纸纤维素酶、漆酶、过氧化物酶的活性。结果表明:在葡萄糖和酵母膏配制比中,羧甲基纤维素酶活性、半纤维素酶活性、淀粉酶活性、漆酶活性在葡萄糖和酵母膏配比为2∶1处理下最强;滤纸纤维素酶活性在葡萄糖和酵母膏配比为7∶2处理下最强;过氧化物酶活性在葡萄糖和酵母膏配比为7∶1处理下最强,其中葡萄糖和酵母膏配比为2∶1处理与配比为7∶2处理的滤纸纤维素酶活性差异不显著。在选用葡萄糖作为碳源、酵母膏为氮源时,基础培养基中碳源和氮源最适配比为2∶1,是适合猴头菌生长的最适碳氮配比。In order to reveal the effect of the ratio of carbon source and nitrogen source on the extracellular enzymes activity of Hericium erinaceus,glucose was used as carbon source,yeast extract as nitrogen source,Hericium erinaceus strain RT22 as material,and the ratio of glucose to yeast extract in basic medium was 3∶1 as control.The ratio of glucose to yeast extract in basic medium was 5∶3,2∶1,7∶3,5∶2,7∶2,5∶1,6∶1,7∶1 as control.After 8 days of shaking culture,the activity of carboxymethyl cellulase,hemicellulase,amylase,filter paper cellulase,laccase and peroxidase were determined.The results showed that carboxymethyl cellulase activity,hemicellulase activity,amylase activity and laccase activity were the strongest in the ratio of glucose to yeast extract at 2∶1,cellulase activity of filter paper was the strongest in the ratio of glucose to yeast extract at 7∶2,and peroxidase activity was the strongest in the ratio of glucose to yeast extract at 7∶1.The cellulase activity of filter paper treated with 2∶1 medium glucose and yeast extract and 7∶2 medium glucose had no significant difference.When glucose was used as carbon source and yeast extract as nitrogen source,the optimum ratio of carbon source and nitrogen source in the basic medium was 2∶1,which was the optimum ratio of carbon and nitrogen for the growth of Hericium erinaceus.

关 键 词：猴头菌 胞外酶 碳氮比 

分 类 号：S646[农业科学—蔬菜学]