全反式维甲酸对肝癌细胞系HepG2中肿瘤干细胞相关基因蛋白表达的影响  

Effect of all-transretinoic acid on tumor stem cells related genes protein expression in liver cancer cell line HepG2

作　　者：朱新锋 张霞 白建华[2] 高红强[2] ZHU Xinfeng;ZHANG Xia;BAI Jianhua;GAO Hongqiang(Department of Hepatopancreatobiliary Surgery,Affiliated Wuhan Central Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430000,China;Department of Hepatopancreatobiliary Surgery,Affiliated Ganmei Hospital,Kunming Medical University,Kunming,Yunnan 650011,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院肝胆胰外科,武汉430000 [2]昆明医科大学附属甘美医院肝胆胰外科,昆明650011

出　　处：《重庆医学》2019年第5期733-737,共5页Chongqing medicine

基　　金：云南省应用基础研究[(昆医联合专项)2013FZ2]

摘　　要：目的探讨全反式维甲酸(ATRA)对肝癌细胞系HepG2中肿瘤干细胞相关基因蛋白表达的影响。方法选取肝癌细胞系HepG2细胞株行传代培养后,分选CD133+的HepG2肝癌干细胞接种于96孔板,分为高量组、低量组、对照组,每组32孔。高量组加入10μmol/L ATRA,低量组加入1μmol/L ATRA,对照组不作任何处理。通过细胞增殖实验(MTT法)检测细胞增殖能力,通过蛋白质印迹法检测Cyclin D和STAT3基因蛋白水平,统计分析所有HepG2肝癌干细胞的细胞增殖能力、Cyclin D和STAT3基因蛋白水平。结果耐药型肝癌HepG2细胞在1、5、10d时细胞增殖能力方面,高量组、低量组、对照组呈逐渐上升趋势,但高量组上升值明显低于低量组,低量组上升值明显低于对照组,差异有统计学意义(P<0.05);蛋白质印迹法ATRA的1、5、10d结果表明,与对照组比较,高量组、低量组Cyclin D和STAT3基因蛋白表达水平不断下降,但高量组下降值明显高于低量组,差异有统计学意义(P<0.05)。结论 ATRA可有效降低肝癌细胞系HepG2中肿瘤干细胞Cyclin D、STAT3等基因蛋白表达水平,有利于抑制HepG2肝癌干细胞的增殖。Objective To discuss the effect of all-transretinoic acid(ATRA)on tumor stem cells related genes protein expression in liver cancer cell line HepG2.Methods The liver cancer cell line HepG2 was selected for conducting the passage culture.The CD133+HepG2 liver cancer stem cells were seeded on 96 well plates,which were divided into the high level group,low level group and control group,32 holes in each group.The high level group was added with 10μmol/L ATRA,the low level group was added with 1μmol/L ATRA and the control group had no any processing.The cell proliferation ability was detected by using the MTT method,Western blot method was used to detect Cyclin D and STAT3 gene protein levels.Then the proliferation ability of HepG2 liver cancer stem cell,Cyclin D and STAT3 gene protein levels were statistically analyzed.Results In the aspect of A570 value of drug-resistant type liver cancer HepG2 cells on 1,5,10 d,the high level group,low level group and control group showed the gradual increasing trend,but the rising value in the high level group was significantly lower than that in the low level group,and the rising value in the low level group was significantly lower than that in the control group,and the differences were statistically significant(P<0.05).The ATRA results detected by Western blot on 1,5,10 d indicated that compared with the control group,the cyclin D and STAT3 gene protein expression levels in the high level group and low level group were constantly decreased,but the descend value in the high level group was significantly higher than that in the low level group,and the difference was statistically significant(P<0.05).Conclusion ATRA can effectively reduce the Cyclin D and STAT3 gene protein expression levels in liver cancer stem cell line HepG2,and is helpful to reduce the proliferation of liver cancer stem cells HepG2.

关 键 词：全反式维甲酸 耐药型 肝癌干细胞 HEPG2 增殖 

分 类 号：R735.7[医药卫生—肿瘤]