人肾透明细胞癌荷瘤裸鼠成瘤细胞浓度检测及相关研究  

Determination of tumorigenic cell concentration in tumor-bearing nude mice with human renal clear cell carcinoma and related studies

作　　者：张鲲鹏 邹泓[1] Zhang Kunpeng;Zou Hong(School of Medicine,Shihezi University,Shihezi,Xinjiang 832002,China)

机构地区：[1]石河子大学医学院

出　　处：《石河子大学学报（自然科学版）》2018年第6期675-679,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(81460383)

摘　　要：目的探讨肾透明细胞癌荷瘤裸鼠最佳成瘤细胞浓度,优化肾透明细胞癌裸鼠成瘤模型。方法根据查询文献设定细胞梯度,连续传代肾透明细胞癌癌细胞(786-0,ACHN),裸鼠前肢腋下注射成瘤,记录观察成瘤时间和大小,显微镜下观察肿瘤的形态及坏死状况,分析最佳成瘤细胞浓度。结果细胞注射后观察至第四周末(裸鼠处死前),1×106/点组裸鼠未成瘤,其他各组自第10天左右开始成瘤,4周后肿瘤最小1.01cm3,最大1.36cm3,肿瘤随注射细胞浓度的增加而增大,最大肿瘤表皮破溃,肿瘤切面肉眼观肿瘤液化坏死与注射浓度呈明显的正相关。组织学显示肿瘤坏死程度与肉眼观相似。结论肾透明细胞癌成瘤裸鼠最佳成瘤细胞浓度为3×106/点(786-0,ACHN),在一定的范围内(3×106~10×106),随细胞注射浓度的增加,肿瘤生长越快。Objective To explore the optimal concentration and experience of tumorigenic cells in the tumor-bearing nude mice of renal clear cell carcinoma, and to optimize the tumorigenic model of the nude mice of renal clear cell carcinoma. Methods According to literature query, cell gradient was set up, and a large number of renal clear cell carcinoma cells (786-0, ACHN) were cultured and then tumor cells were injected into nude mice forelimbs underarm. Time and tumor size, morphology and necrosis of tumor were observed under microscope to analyze the best tumor formation of concentration into tumor cells. Results Cell injection was observed until the fourth weekend (before the execution of nude mice), no tumorigenesis was observed in nude mice (1×106/ point), the other groups started to develop tumors around day 10, and tumor stripped after 4 weeks. The tumor size raged from minimum 1.01 cm2 to maximum 1.36 cm2. The tumor increased in size with the increase of the injection cell concentration, neoplasm grossly tumor liquefaction necrosis and obvious positive correlation to the concentration of injection. Histology showed that the degree of tumor necrosis was similar to the visual observation. Conclusion The best tumorigenic cell concentration in nude mice with renal clear cell carcinoma(786-0, ACHN) was 3×106/ point . Within the set range (3×106-10×106), the tumor grew faster with the increased cell injection concentration.

关 键 词：CD138/Syndecan-1 软组织肉瘤 脂肪肉瘤 免疫组织化学 

分 类 号：R73.354[医药卫生—肿瘤]