血管生成拟态与Hif-1α、EphA2在原发性肝癌中表达关系的研究  

A pilot study of the expression relationship of VM,Hif-1α and EphA2 in primary liver cancer

作　　者：李杰[1] 马晓洁[2] 杜小波[1] 谭榜宪[2] LI Jie;MA Xiao-jie;DU Xiao-bo;TAN Bang-xian(Department of Oncology,1.Mianyang Central Hospital,Mianyang 621000;Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]绵阳市中心医院肿瘤科,四川绵阳621000 [2]川北医学院附属医院肿瘤科,四川南充637000

出　　处：《川北医学院学报》2019年第1期36-39,共4页Journal of North Sichuan Medical College

基　　金：四川省教育厅重点项目(10ZA077)

摘　　要：目的:探讨原发性肝癌(primaryliver cancer,PLC)中血管生成拟态(vasculogenic mimicry,VM)与缺氧诱导因子-1α(hypoxia inducible factor-1α,Hif-1α)、上皮细胞激酶(epithelial cell kinase,EphA2)的关系,阐述VM的形成机制。方法:患者临床病理资料完整的PLC术后蜡块标本161例,采用CD31-PAS双染法观察VM的表达。免疫组织化学染色观察Hif-1α和EphA2的表达。结果:VM在PLC中的阳性表达率为37.9%,与肿瘤大小、Edmondson分级、临床分期呈正相关(P<0.05);肝硬化组VM阳性率为55.6%,明显高于非肝硬化组31.0%(χ~2=8.284,P=0.004);VM阳性组和阴性组Hif-1α、EphA2表达差异有统计学意义(χ~2=10.608,P=0.014;χ~2=8.490,P=0.037)。结论:VM在PLC中存在表达,肿瘤体积大、临床分期晚、细胞分化程度低形成VM的能力强,肝硬化能增强形成VM的能力;Hif-1α和EphA2的表达可能促进VM形成。Objective:Explore the expression relationship of VM,Hif-1α and EphA2 in Primary liver cancer and further to investigate the potential mechanism of VM.Methods:The complete clinical and pathological data of 161 PLC cases were collected in this study.The expression of VM were observed by immunohistochemical double staining of CD31-PAS,and detected the expression of Hif-1α and EphA2 proteins by immunohistochemical staining.Results:The positive rates of VM in PLC was 37.9%.The expression of VM was positively correlated with TNM stage,tumor size and HCC Edmondson stage(P﹤0.05).The positive rate of VM in cirrhosis group was 55.6%,which was significantly higher than that in non-cirrhosis group(31%,χ^2=8.284,P=0.004).There were significant differences in the expression of Hif-1α and EphA2 between VM positive and negative groups(χ^2=10.608,P=0.014;χ^2=8.490,P=0.037).Conclusion:VM exists in primary liver cancer.The later TNM stage of PLC,the higher pathological grade(the lower degree of cell differentiation) lead to a higher expressive ability of VM,and PLC with cirrhosis of the liver can increase the expression.Hif-1α and EphA2 are important regulatory factor in the formation of the VM.

关 键 词：血管生成拟态(VM) 原发性肝癌 缺氧诱导因子-1α(Hif-1α) 上皮细胞激酶(EphA2) 

分 类 号：R735.7[医药卫生—肿瘤]