鸭疫里默氏杆菌对鸭胚肝细胞粘附入侵能力的研究  

ADHESION AND INVASION OF RIEMERELLA ANATIPESTIFER TO DUCK EMBRYO LIVER CELLS

作　　者：薛亚飞[1] 宫艳杉 祁晶晶[1] 卜慧敏 杨永胜 陈妍 高萌萌 胡青海[1] XUE Ya-fei;GONG Yan-shan;QI Jing-jing;BO Hui-min;YANG Yong-sheng;CHEN Yan;GAO Meng-meng;HU Qing-hai(Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China)

机构地区：[1]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2019年第1期37-41,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(31772770;31472224;31072156;31272590);上海市自然科学基金(15ZRl449700);上海闵行领军人才专项资金

摘　　要：为了分析鸭胚肝细胞用于鸭疫里默氏杆菌粘附入侵实验的可行性,本研究比较了鸭疫里默氏杆菌对鸭胚肝细胞和Vero细胞的粘附和入侵能力。首先将构建的互补质粒pRES1-ompA通过双亲本接合转导的方法导入ompA基因缺失株Th4ΔompA中,构建得到缺失株的回复菌株cTh4ΔompA;然后比较了野生株Th4、缺失株Th4ΔompA及回复株cTh4ΔompA菌株对鸭胚肝细胞和Vero细胞的粘附入侵能力。结果表明,与野生株Th4菌株相比,缺失株Th4ΔompA对鸭胚肝细胞和Vero细胞的粘附能力分别降低了3.32倍和3.12倍,入侵能力分别降低了2.25倍和7.58倍,而cTh4ΔompA对鸭胚肝细胞和Vero细胞的粘附入侵能力均得到部分回复。本研究结果表明鸭胚肝细胞可用于鸭疫里默氏杆菌粘附入侵能力的分析。The objective of the present study was to investigate whether or not duck embryo hepatocytes could be used to study adhesion and invasion capabilities of Riemerella anatipestifer. The complemented plasmid pRESl-ompA was introduced into the mutant Th4 AompA by conjugation to generate the rescued strain cTh4AompA. Then adhesion and invasion abilities of the parent wild strain Th4, mutant strain Th4AompA and complemented strain cThAhompA were studied by inoculating duck embryo hepatocytes and Vero cells. The result showed that the mutant strain Th4AompA decreased their adhesion and invasion abilities by 3.32-fold and 2.25-fold to embryo hepatocytes, and 3.12-fold and 7.58-fold to Vero cells as compared with the wild type Th4. In contrast, the adhesion and invasion capacities of the complemented strain cThAAompA were partially recovered in duck embryo hepatocytes. The results indicated that duck embryo hepatocytes might be used to analyze the capacity of adhesion and invasion of R. anatipestifer.

关 键 词：鸭疫里默氏杆菌 鸭胚肝细胞 VERO细胞 粘附 入侵 

分 类 号：S852.612[农业科学—基础兽医学]