苦瓜Ⅰ型核糖体失活蛋白对膀胱癌迁移能力的影响及机制研究  

Effects and mechanism of Momordica charantia MAP30 on migration of bladder cancer

作　　者：庞昆[1] 陈波[1] 郝林[1] 史振铎[1] 周荣升[1] 臧光辉[1] 韩从辉[1] Pang Kun;Chen Bo;Hao Lin;Shi Zhenduo;Zhou Rongsheng;Zang Guanghui;Han Conghui(Department of Urology, Xuzhou Central Hospital Affiliated to Southeast University, Xuzhou 221000, China)

机构地区：[1]东南大学附属徐州市中心医院泌尿外科,221000

出　　处：《中国医师杂志》2019年第2期184-188,共5页Journal of Chinese Physician

基　　金：国家自然科学基金(81774089);江苏省中医药局科技项目(YB2017055);江苏省高等学校自然科学研究面上项目(17KJB360001);江苏省医学创新团队培养(CXTD-2016-48)~~

摘　　要：目的研究苦瓜Ⅰ型核糖体失活蛋白(MAP30)对膀胱癌迁移能力的影响及机制。方法采用MTT法计算MAP30对人膀胱癌5637和T24细胞的半数抑制浓度(IC50)后,采用此浓度的MAP30通过细胞划痕迁移实验和Transwell细胞迁移实验来评估两种细胞的迁移能力,并用Western blot分别检测两种肿瘤细胞加入MAP30后基质金属蛋白酶(MMPs)和黏附分子N-cadherin等蛋白的表达。结果细胞划痕迁移实验:5637细胞加药组和对照组在8 h和22 h迁移率比较差异均有统计学意义(P <0. 05),T24细胞加药组和对照组间8 h迁移率差异无统计学意义(P> 0. 05),在22 h迁移率差异有统计学意义(P <0. 05)。MAP30干预后,人膀胱癌5637细胞和T24细胞中Vimentin、Fibronectin、MMP-2、MMP-9、N-Cadherin蛋白的表达明显降低。人膀胱癌5637细胞E-Cadherin表达降低,但人膀胱癌T24细胞未检测到目的条带。结论苦瓜Ⅰ型核糖体失活蛋白MAP30通过抑制上皮-间质细胞转化(EMT)途径和抑制MMPs表达,可以有效抑制人膀胱癌5637和T24细胞的迁移能力。Objective To study the effect and mechanism of Momordica anti-HIV protein of 30 ku (MAP30) on the migration of bladder cancer. Methods The IC50 of human bladder cancer 5637 and T24 cells was calculated by methyl thiazolyl tetrazolium (MTT) method. The migration ability of these two cells was evaluated by scratch migration test and Transwell cell migration test. The expression of migrating proteins such as matrix metalloproteinases (MMPs) and adhesion molecule N-cadherin were compared by Western blot. Results Scratch migration test: there were significant differences in migration rates of 5637 cells at 8 h and 22 h (P<0.05). There were significant differences in migration rates of T24 cells at 22 h (P<0.05), but no significant differences in migration rates at 8 h (P>0.05). The expression of Vimentin, Fibronectin, MMP-2, MMP-9 and N-Cadherin in 5637 cells and T24 cells of human bladder cancer decreased significantly after adding MAP30. The E-Cadherin expression in human bladder cancer 5637 cells were decreased, but no target band was detected in human bladder cancer T24 cells. Conclusions The ribosome-inactivating protein MAP30 can effectively inhibit the migration of human bladder cancer 5637 and T24 cells by inhibiting the EMT pathway and inhibiting the expression of MMPs.

关 键 词：核糖体失活蛋白质类 1型 膀胱肿瘤 细胞迁移分析 上皮细胞-间质转化 体外研究 

分 类 号：R737.14[医药卫生—肿瘤]