阿霉素通过两种方式诱导乳腺癌耐药细胞株的比较研究  

Comparison of adriamycin-resistant breast cancer lines induced in two ways

作　　者：李晓玲 张莉 Li Xiaolin;Zhang Li(Department of Oncology,Renmin Hospital of Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属人民医院肿瘤中心,十堰442000

出　　处：《中国医师杂志》2019年第2期194-196,共3页Journal of Chinese Physician

基　　金：湖北省教育厅科学技术研究项目(B2016139)~~

摘　　要：目的通过两种不同方式诱导乳腺癌耐药细胞株(MCF-7/ADM),比较两种诱导方式的生物学特性。方法以乳腺癌MCF-7细胞株为亲本细胞,采用低浓度持续递增法和高浓度冲击递增法分别诱导乳腺癌耐药细胞株MCF-7/ADM1和MCF-7/ADM2;显微镜下观察细胞形态,MTT法检测不同浓度ADM对MCF-7、MCF-7/ADM1和MCF-7/ADM2的抑制率,计算耐药指数(RI);绘制生长曲线,计算细胞倍增时间(Td);经不同浓度阿霉素(ADM)冻存后检测RI变化;流式细胞仪检测细胞周期。结果采用低浓度持续递增法和高浓度冲击递增法成功诱导了乳腺癌耐药细胞株,且对紫杉醇和顺铂产生了交叉耐药性; MCF-7/ADM1的RI高于MCF-7/ADM2(P <0. 05);三种细胞株Td分别为:24. 01、34. 04、32. 71 h。两种耐药细胞株冻存后RI变化不明显(P> 0. 05)。与亲本细胞比较,耐药细胞株S、G2/M期细胞减少,G0/G1期细胞增多。结论两种方式均可诱导耐药细胞株,且其耐药性相对稳定,并可对紫杉醇及顺铂产生交叉耐药,但其生物学特性有所差异,有待进一步研究其耐药机制及逆转靶点。Objective To compare the biological characteristics of two Adriamycin-resistant breast cancer lines induced in different methods. Methods The MCF-7/ADM1 and MCF-7/ADM2 of breast cancer resistant cell lines were induced by low-concentration continuous increasing method and high concentration impact increasing method respectively.The morphological characteristics of the two cell lines were observed microscopically. The resistance index of the cells was determined with methyl thiazolyl tetrazolium (MTT) assay. The cell growth curves were drawn, and calculate the cell double time. Cryopreservation in different concentrations of adriamycin (ADM), the resistant index (RI) changes were detected and the cell cycle was detected by flow cytometry. Results We succeeded in inducing drug-resistant breast cancer cell lines, which were cross-resistance to paclitaxel and cisplatin. The RI of MCF-7/ADM1 was higher than that of MCF-7/ADM2 (P<0.05). The Td of three cell lines were 24.01 h, 34.04 h and 32.71 h, respectively. The RI of the two drug-resistant cell lines did not change significantly after cryopreservation (P>0.05). Compared with MCF-7 cell line, cells of drug-resistant cell lines decreased in S, G2/M phase, while that in G0/G1 phase increased. Conclusions The two methods can induce drug-resistant cell lines, and the resistance was relatively stable, but its biological characteristics were partially different. Its resistance mechanism and reversal target need to be further studied.

关 键 词：乳腺肿瘤 抗药性 多柔比星 体外研究 

分 类 号：R737.9[医药卫生—肿瘤]