不同浓度麝香含药血清对骨髓间充质干细胞迁移影响的实验研究  

Experimental study on the effects of different concentrations of musk containing serum on the migration of bone marrow mesenchymal stem cells

作　　者：李应福 谢兴文[1,2] 李宁[3] 侯费祎[4] 蒋国鹏 潘鑫戊 周文杰[3] LI Yingfu;XIE Xingwen;LI Ning;HOU Feiyi;JIANG Guopeng;PAN Xinwu;ZHOU Wenjie(Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050;Gansu Provincial Institute of TCM , Lanzhou 730050;Gansu University of TCM , Lanzhou 730000;General Hospital of Lanzhou Military Command, Lanzhou 730050, China)

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]甘肃省中医药研究院,甘肃兰州730500 [3]甘肃中医药大学,甘肃兰州730000 [4]兰州军区总医院,甘肃兰州730050

出　　处：《中国骨质疏松杂志》2019年第2期156-162,共7页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81460735);国家中医药管理局中医临床研究基地业务建设科研专项(JDZX2015080)

摘　　要：目的探讨麝香对外源性骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖和迁移的影响。方法将60只SD大鼠随机分为麝香高、中、低剂量组及空白对照组,制备麝香含药血清及生理盐水血清。15只SD大鼠利用全骨髓贴壁法分离BMSCs,培养至P3代,通过形态学观察、表型鉴定、成骨成脂诱导鉴定BMSCs,鉴定认为培养成功后通过麝香含药血清干预BMSCs,检测细胞增殖率,利用Transwell实验检测麝香含药血清对BMSCs迁移的影响。结果外源性大鼠BMSCs呈梭形贴壁生长,生长状态良好;表型鉴定:CD45、CD34阴性表达,CD44、CD90阳性表达;细胞成骨、成脂诱导后可定向成骨、成脂分化;不同浓度麝香组与对照组比较均能提高BMSCs增殖率(P<0.05);与对照组比较,不同浓度麝香在24 h、48 h、72 h均增加BMSCs迁移(P<0.05),以低浓度组效果最佳。结论麝香含药血清可以促进BMSCs增殖,促进BMSCs的体外迁移。Objective To investigate the effects of musk on the proliferation and migration of exogenous bone marrow mesenchymal stem cells(BMSCs). Methods Sixty SD rats were randomly divided into high, medium and low dose musk group and blank control group, and the control serum and musk containing serum were prepared. BMSCs were isolated from fifteen SD rats by whole bone marrow adherence method, which was then cultured to P3 generation. BMSCs were identified by morphological observation, phenotypic identification, and osteogenic and adipogenic induction. After identifying the success of cell culture, BMSCs were intervened by musk containing serum, then the cell increment rate was detected, and Transwell assay was used to detect the effect of musk serum on the migration of BMSCs. Results Exogenous rat BMSCs showed fusiform adherent growth, with good growth state. Phenotypic identification: the expression of CD45 and CD34 were negative, the expression of CD44 and CD90 were positive. After osteogenic and adipogenic induction, the cells could be induced into osteogenic and adipogenic differentiation. Compared with the control group, musk groups of different concentrations could increase the proliferation rate of BMSCs(P<0.05);compared with the control group, different concentrations of musk increased BMSCs migration at 24 h, 48 h, 72 h(P<0.05), with the best effect in the low concentration group. Conclusion Musk serum can promote the proliferation of BMSCs and promote the migration of BMSCs in vitro.

关 键 词：麝香 骨髓间充质干细胞 增殖 迁移 大鼠 动物实验 

分 类 号：R-332[医药卫生]