四川省广元市胃肠炎暴发中诺如病毒的分子病原学特征研究  

Molecular etiological characteristics of the gastroenteritis outbreak associated with norovirus infection in Guangyuan city, Sichuan province

作　　者：侯雪芹[1] 任敏[2] 熊丽 朱俊[1] 曹冉冉[2] 马小珍[2] Hou Xueqin;Ren Min;Xiong Li;Zhu Jun;Cao Ranran;Ma Xiaozhen(Guangyuan Center for Disease Control and Prevention,Guangyuan 628000,China;Sichuan Center for Disease Control and Prevention,Chengdu 610041,China)

机构地区：[1]广元市疾病预防控制中心,628000 [2]四川省疾病预防控制中心,成都610041

出　　处：《中华实验和临床病毒学杂志》2019年第1期39-43,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解四川省广元市急性胃肠炎(acute gastroenteritis,AGE)暴发疫情中诺如病毒(Norovirus,NoV)感染情况,明确病原基因型别,并对其基因特征进行初步研究。方法采集广元市2017年2月至5月4起AGE疫情病例的粪便标本18份、肛拭子标本15份,采用实时荧光RT-PCR检测NoV基因,NoV阳性标本用传统RT-PCR扩增并进一步测序分型。结果NoV实时荧光RT-PCR结果显示4起疫情均为NoV感染引起的暴发,检测阳性率为60.6%(20/31)。基因序列比对和进化分析结果显示,4起疫情的病原均为GⅡ.P16/GⅡ.2型NoV,且核苷酸高度同源,其中1起除GⅡ.P16/GⅡ.2型外还检出GⅡ.P7/GⅡ.14型NoV。结论四川省广元市4起AGE暴发疫情主要由GⅡ.P16/GⅡ.2型NoV引起,同时存在GⅡ.P7/GⅡ.14型NoV混合感染。Objective To investigate the genotype and genetic characteristics of the pathogens associated with the epidemic outbreak of acute gastroenteritis(AGE) in Guangyuan city,Sichuan province.Methods Eighteen stool samples and 15 anal swab samples from 4 AGE outbreaks were collected from Feb 2017 to May 2017.Norovirus (NoV) nucleic acid was detected by Real-time PCR method,and the positive samples were amplified by conventional reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing.Results Four AGE outbreaks were all caused by NoV.And 20 (60.6%) samples were positive for NoV G E.Gene sequence comparison and phylogenetic analysis showed that 4 AGE outbreaks were all caused by GⅡ.P16/GⅡ.2 NoV.All the strains of GⅡ.P16/GⅡ.2 NoV shared high homology in nucleotides.One of the outbreaks was caused by G E.P16/GⅡ.2 and GⅡ.P7/GⅡ.14 NoV co-infection.Conclusions The 4 outbreaks of AGE in Guangyuan city,Sichuan province were major caused by GⅡ.P16/GⅡ.2 NoV,meanwhile GⅡ.P16/GⅡ.2 and GⅡ.P7/GⅡ.14 NoV co-infection existed.

关 键 词：诺如病毒 胃肠炎 暴发 系统进化分析 

分 类 号：R512.5[医药卫生—内科学] R181.3[医药卫生—临床医学]