髓样细胞触发受体-1基因rs9471535单核苷酸多态性与男性早发冠心病的相关性  

Relationships between TREM-1 gene polymorphism and susceptibility to male premature coronary artery disease

作　　者：杨勇[1] 孟涛疆 马东星 刘英[1] 沈志奇 黄洁[1] 赵清 王冉冉[1] 杨长春 YANG Yong;MENG Taojing;MA Dongxin;LIU Ying;SHEN Zhiqi;HUANG Jie;ZHAO Qing;WANG Ranran;YANG Changchun(Department of Cardiology, the Third Medical Center of PLA General Hospital, Beijing 100039 , China;SanyaRehabilitation Center of PLA Joint Logistics Support Force, Sanya 572000, China)

机构地区：[1]解放军总医院第三医学中心心血管内科,北京100039 [2]解放军联勤保障部队二亚康复疗养中心,572000

出　　处：《武警医学》2019年第2期97-100,104,共5页Medical Journal of the Chinese People's Armed Police Force

基　　金：原武警总医院科研创新预研资助项目(WZ2015013).

摘　　要：目的探讨髓样细胞触发受体-1(TREM-1)基因rs9471535单核苷酸多态性(SNP)与男性早发冠心病(PCAD发病相关性。方法收集原武警总医院心内科2015-08至2017-01收治的男性PCAD住院患者(男性<55岁)及同期年龄匹配的健康对照者(经冠状动脉造影或冠状动脉CTA排除冠心病)纳入研究。通过聚合酶链式反应及连接酶检测反应(PCR-LDR)技术对TREM-1基因rs9471535单核苷酸多态性位点进行基因分型,比较PCAD与对照组两组间等位基因频率并与PCAD发生风险之间进行关联性分析。结果 PCAD组高血压(57. 0%)、糖尿病(21. 5%)、高脂血症(59. 5%)、吸烟史(74. 4%)患者比例明显高于对照组(29. 1%,10. 7%,15. 8%,32. 1%),组间差异有统计学意义(P <0. 05);在PCAD组中rs9471535位点基因型分布为TT(46. 3%)、CT(50. 4%)、CC(3. 3%),等位基因频率为T(71. 5%)、C(28. 5%);在对照组中的基因型分布为TT(51. 0%)、CT(38. 3%)、CC(10. 7%),等位基因频率为T(72. 7%)、C(27. 3%),基因型组间分布有统计学差异(P=0. 017);经二元logistic回归单因素分析表明,与TT基因型相比,rs9471535位点CC基因型在PCAD组与对照组间的分布无统计学差异(P=0. 059,OR=0. 340,95%CI=0. 111~1. 041);多因素分析结果显示rs9471535位点CC基因型在PCAD组与对照组间的分布有统计学差异(P=0. 011,OR=0. 180,95%CI=0. 048~0. 679),提示携带CC基因型的男性PCAD人群的发病风险比携带TT基因型的人群低。结论 TREM-1基因rs9471535位点多态性可能与男性PCAD的发病相关,携带CC基因型的人群患PCAD的风险可能较携带TT基因型的人群低,CC基因型可能是男性PCAD的保护因素。Objective To explore the correlations l)etween gene polymorphisms of triggering receptor expressed on myeloid cells-1 (TREM-1) rs9471535 and susceptibility to male premature coronary artery disease ( PCAD). Methods Between August 2015 and January 2017, male inpatients aged 55 or above, treated at the Department of Cardiology of our hospital, and identified by coronary angiography or CT A with CAD (PCAD group) or without CAD (control group), were enrolled into this study. The DNA kit was used to extract DNA from peripheral venous blood, and polymerase chain reaction-Iigase detection reaction (PCR-IQR) technology was used to identify the TREM-1 gene polymorphisms. The frequencies of genotypes and alleles of rs9471535 were calculated and binary logistic regression was used to analyze the relationships between rs9471535 polymorphisms and susceptibility to PCAD. Results The percentages of patients with hypertension (57.0%), diabetes (21.5%), hyperlipidemia (59. 5%) or with smoking history (74.4%) were significantly higher in the PCAD group than those in the control group (P vO.05). The frequencies of genotypes TT(46. 3%), CT (50.4%)and CC (3. 3%)of rs9471535 in the PCAD group were significantly different from those of the control group, which were 51.0%, 38. 3%,and 10. 7% respectively (P = 0.017). According to univariate analysis with binary logistic regression, as compared with TT genotype, the distribution of CC genotype was not signif-icantly different between the two groups (P = 0.059,OR =0. 340,95% CI =0. 111-1. 041 ). However, after adjustment of traditional risk factors (age, hypertension, diabetes, hyperlipidemia and smoking), carriers of CC genotype had lower risk of PCAD (P =0.011=0. 180,95%CI = 0.048-0.679). Conclusions The TREM-1 gene rs9471535 polymorphism may have correlations with the occurrence of niale premature CAD. Individuals with CT and TT genotypes may be more vulnerable to premature CAD than those carrying CC genotype that may play a protective role in male PCAD.

关 键 词：早发冠心病 TREM-1基因 rs9471535 单核苷酸多态性 

分 类 号：R394.5[医药卫生—医学遗传学]