高压氧介导的脑内Th17/Treg细胞比例调控对阿尔茨海默病小鼠模型中淀粉样蛋白的作用研究  

Effects of hyperbaric oxygen on Th17/Treg cell infiltration in the brain tissue of Alzheimer disease mice

作　　者：孙银峰[1] 傅敏[1] 童蓓 付剑亮[1] Sun Yinfeng;Fu Min;Tong Bei;Fu Jianliang(Department of Hyperbaric Oxygen,Shanghai No.6 Peoples Hospital Afriliated to Shanghai Jiao Tong University,Shanghai 200233,China)

机构地区：[1]上海交通大学附属第六人民医院高压氧科,上海200233

出　　处：《中华航海医学与高气压医学杂志》2019年第1期47-52,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨高压氧治疗对阿尔茨海默病(Alzheimer disease,AD)小鼠脑组织中Th17/Treg细胞浸润的影响及其作用机制。方法APP/PS1双重转基因小鼠给予高压氧治疗后免疫荧光染色和Western blotting检测脑组织中Aβ的沉积,流式细胞术检测脑组织中Th17细胞与Treg细胞的浸润情况。结果高压氧治疗后的AD小鼠脑组织中Aβ沉积显著减少,差异有统计学意义(P<0.01);另外,高压氧治疗使AD小鼠脑组织中IL-17升高、而FOXP3的表达却显著下降,差异均有统计学意义(均P<0.01);高压氧治疗可上调AD小鼠脑组织中的Th17/Treg细胞比例(P<0.01)。结论高压氧治疗可以通过上调AD小鼠脑组织中的Th17/Treg细胞比例,减低AD小鼠脑组织中Aβ的沉积,为高压氧治疗AD提供了实验依据。Objective To explore the effects of hyperbaric oxygen(HBO)on Th17/Treg cell infiltration in the brain tissue of Alzheimer disease(AD)mice,and also to identify possible mechanism involved for the improvement of AD conditions.MethodsFollowing HBO treatment of APP/PS1 double transgenic mice,immunofluorescence staining and Western blotting were used to detect the deposition of Aβin the brain tissue.Flow cytometry was used to analyze the infiltration of Th17 cells and Treg cells in the brain tissue.ResultsThe deposition of Aβin the brain tissue of AD mice after HBO therapy was significantly reduced(P<0.01).In addition,HBO would increase the expression of IL-17,while it could significantly decrease the expression of FOXP3(P<0.01).Flow cytometry detection results indicated that HBO therapy could up-regulate the proportion of Th17/Treg cells in the brain tissue of AD mice(P<0.01).ConclusionHBO could decrease Aβdeposition in the brain tissue through up-regulation of the proportions of Th17/Treg cells in the brain tissue of AD mice.

关 键 词：高压氧 阿尔茨海默病 Th17细胞 TREG细胞 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]