雷公藤致大鼠肝脏、肾脏和心脏组织中代谢变化的研究  

Metabolic changes in liver, kidney and heart of rats induced by Tripterygium wilfordii administration

作　　者：陈连国 姜燕燕 金伟强 温从丛 陈善浆 林崇良[4] CHEN Lian-guo;JIANG Yan-yan;JIN Wei-qiang;WEN Cong-cong;CEHN Shan-jiang;LIN Chong-liang(Pharmacy, Wenzhou People's Hospital, Wenzhou, Zhejiang 325000, China)

机构地区：[1]温州市人民医院药剂科,浙江温州325000 [2]温州医科大学实验动物中心,浙江温州325035 [3]温州市中心医院心血管内科,浙江温州325000 [4]温州医科大学附属第一医院,浙江温州325000

出　　处：《中国卫生检验杂志》2019年第5期542-545,共4页Chinese Journal of Health Laboratory Technology

基　　金：温州市公益性科技计划项目(Y20170281)

摘　　要：目的基于组织代谢组学方法的气相色谱-质谱(GC-MS)来评估雷公藤对大鼠的影响。方法 30只大鼠随机分为雷公藤低剂量组、雷公藤高剂量组和对照组。低剂量组、高剂量组大鼠每天灌胃给药0.5 g/kg和1.0 g/kg的雷公藤,连续7 d;对照组给予生理盐水,连续灌胃7 d。用偏最小二乘法分析(PLS-DA)显示雷公藤诱导代谢扰动。结果与对照组相比,低剂量组大鼠肝脏中十六酸、肾脏中d-核糖以及心脏中d-葡萄糖含量均升高,肾脏和心脏中十六酸含量均降低;高剂量组大鼠肾脏中L-苏氨酸含量和心脏中L-脯氨酸、9H-嘌呤、十六酸、L-脯氨酸含量与对照组相比均降低。结论 PLS-DA结果表明,GC-MS代谢组学方法可以区分对照组和雷公藤组大鼠代谢情况的差异。代谢物的增加或减少表明雷公藤可诱导大鼠能量代谢、氨基酸代谢和脂质代谢紊乱。Objective In this study, we developed a tissue metabolomic method by gas chromatography-mass spectrometry(GC-MS) to evaluate the effect of Tripterygium wilfordii(TW) on rats. Methods Thirty rats were randomly divided into TW groups(the higher dose group and the lower dose group) and control group. The rats in TW groups were respectively given 0.5 g/kg(low dose) and 1.0 g/kg(high dose) of TW by intragastric administration each day for continuous 7 days. The control group was given normal saline for 7 days. Results Partial least squares-discriminate analysis(PLS-DA) revealed that TW induced metabolic perturbations in rats. Compared to the control group, hexadecanoic acid, d-ribose and d-glucose increased in livers, kidneys and hearts of rats, respectively;and the hexadecanoic acid decreased in both kidneys and hearts of rats in lower dose group. Compared to the control group, L-threonine decreased in kidneys, and L-proline, 9 H-purine and L-proline decreased in hearts of rats in high dose group. Conclusion PLS-DA results indicated that metabolomic method by GC-MS could differentiate these rats in the control group and TW groups. The changes of metabolites(increased or decreased) can indicate that TW could induce energy metabolism, amino acid metabolism and lipid metabolism perturbations in tissues of rats.

关 键 词：代谢组学 气相色谱-质谱 雷公藤 组织 大鼠 

分 类 号：R285.5[医药卫生—中药学]