DEC1在肝癌细胞中的表达及分析  

The expression and analysis of differentiated embryo-chondrocyte expressed gene1 (DEC1)in hepatocellular carcinoma cell lines

作　　者：索翠萍[1] 张炳杨 张艳美 郭建壮 石晓红[1] SUO Cui-ping;ZHANG Bing-yang;ZHANG Yan-mei(Department of Laboratory Medicine,Shandong Provincial Qianfoshan Hospital,Shandong Jinan 250014)

机构地区：[1]山东省千佛山医院检验科,山东济南250014

出　　处：《医学检验与临床》2019年第1期37-41,共5页Medical Laboratory Science and Clinics

基　　金：山东省自然科学基金项目(No.ZR20BHL036).

摘　　要：目的:初步探讨肝癌细胞株、正常肝细胞株中DEC1mRNA的表达水平和DEC1蛋白的表达水平和DEC1蛋白亚细胞定位.为进一步发现DEC1与原发性肝癌发生发展的关系提供依据.方法:选取人肝癌细胞株SMMC-7721,HepG-2,Bel-7402,Hep-3B细胞和人正常肝细胞株HL-7702.用RT-PCR、流式细胞术和免疫细胞化学方法检测DEC1mRNA及蛋白的表达水平.结果:其中HL-7702细胞中的DEC1 mRNA表达水平高于其他各肝癌细胞株,但差异没有统计学意义(P>0.05).各细胞株中DEC1蛋白在细胞核与细胞浆均表达阳性.但以HL-7702核表达较强.流式细胞术结果显示DEC1在肝癌细胞株Bel-7402中的标记率为56%.结论:DEC1在各株肝细胞中转录水平表达的差异并不明显.各细胞株中DEC1蛋白在细胞核与细胞浆均表达阳性.Objective: To investigate the expression of differentiated embryo-chondrocyte expressed genel (DEC1) in hepatocellular carcinoma cell lines and estimate functional character of DEC1 in the development of HCC. Methods: 4 kinds of human liver carcinoma cell lines were selected including SMMC-7721, HepG-2, Bel-7402, Hep -3B. Human liver cell line HL-7702 was used as the normal control. Reverse-Transcriptase Polymerase Chain Keaction (KT-PCR) Analysis, Flow cytometry and Immunocytochemistry analysis was used to examine the expression patterns and subcellular location of DEC1. Results: DEC1 mRNA was expressed in all these cell line. Compared with carcinoma cells, expression of DEC1 was stronger in nonnal liver cell, but there is no significance diflerence (P>0.05). In nomial liver cell line HL -7702, and human liver carcinoma cell lines including HepG-2 and Bel-7402, I)EC1 was expressed in the cytoplasm and nueleus. In HL-7702 cell nuclear DEC1 expression is higher than in HepG-2 cells and Bel-7402 cell lines. Conclusion: In the transcription level, there is no remarkably ditlerence among these cell lines. DEC1 protein was expressed in the cytoplasm and nucleus in HCC cells.

关 键 词：DEC1 原发性肝细胞性肝癌 转录因子 

分 类 号：R735.7[医药卫生—肿瘤]