逆转座子LINE1在Hela细胞中的表达及转录组分析  

Expression and transcriptome analysis of retrotransposon LINE1 in Hela cells

作　　者：储旭 周涛 李文豪 邢虎 陈良标[1] CHU Xu;ZHOU Tao;LI Wen-hao;XING Hu;CHEN Liang-biao(National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,International Research Center for Marine Bioscience,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心水产种质资源发掘与利用教育部重点实验室中国科学技术部海洋生物科学国际联合研究中心,上海201306

出　　处：《大连海洋大学学报》2019年第2期153-159,共7页Journal of Dalian Ocean University

基　　金：国家自然科学基金资助项目(31572611);上海市教委水产高峰学科项目

摘　　要：为研究逆转座子LINE1(简称为L1)在Hela细胞内的作用机制,对转染L1的Hela细胞和正常细胞进行转录组测序,通过对转录组数据进行拼接组装,对差异表达基因进行了生物信息学分析。结果表明:试验共筛选出391个差异表达基因,其中45.6%的差异表达基因显著性上调,这些基因通过GO分类注释,可分为14类;通过KEGG对差异基因进行通路富集,表明差异基因主要富集在破骨细胞分化、色氨酸代谢、乙型肝炎、病毒致癌作用和可卡因成瘾等信号通路,发现了一些与细胞黏着连接、表皮相关的基因有差异性表达。研究表明,通过转录组测序,获得了丰富的关于L1在Hela细胞中表达的信息,为进一步研究L1在细胞中的作用机制供了思路和理论依据。Transcriptional sequencing and bioinformatics analysis of retrotransposon LINE1(L1)as a reverse transcriptase-mediated replicative nucleotide element was performed on Hela cells transfected with L1 and wide-type cells to investigate the molecular mechanism of L1 in Hela cells.Bioinformatics analysis showed that a total of 391 differentially expressed genes were screened by splicing and assembling the transcription data,45.6%of which were significantly increased.These genes can be classified into 14 categories through the GO classification annotation.KEGG pathway enrichment indicated that differentially expressed genes mainly enriched in osteoclast differentiation,tryptophan metabolism,hepatitis B,viral carcinogenic and cocaine addiction and some of genes were found to be related to the adhesion and epidermis of the cells.Abundant transcriptome information on the expression of L1 in Hela cells by RNA-seq will provide insight and theoretical basis for further study of L1 mechanism in cells.

关 键 词：LINE1 HELA细胞 转录组测序 逆转座子 

分 类 号：Q291[生物学—细胞生物学]