日粮添加BHT对饲喂氧化豆油黄羽肉鸡肌肉品质和抗氧化能力的影响  

Effects of Dietary Supplementation of BHT on Muscle Quality and Antioxidant Capacity of Yellow Broilers Fed Oxidized Soybean Oil

作　　者：王安谙 葛晓可 张婧菲[1] 冯程程 李思勉 张礼根 张莉莉[1] 王恬[1] WANG An-an;GE Xiao-ke;ZHANG Jing-fei;FENG Cheng-cheng;LI Si-mian;ZHANG Li-gen;ZHANG Li-li;WANG Tian(College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095 ,China)

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《食品工业科技》2019年第6期114-120,125,共8页Science and Technology of Food Industry

摘　　要：本试验旨在研究2,6-二叔丁基-4-甲基苯酚(BHT)对饲喂氧化豆油日粮黄羽肉鸡肌肉品质及抗氧化能力的影响。试验采用2×2因子设计,因素1为日粮中的豆油,按其品质分为新鲜豆油(MDA=13.4 nmol/mL)和氧化豆油(MDA=367.7 nmol/mL),因素2为日粮中BHT含量,按添加剂量分为两水平(0、125 mg/kg)。240羽1日龄优质黄羽肉鸡被随机分成4个处理组,每组6个重复,每个重复10只鸡。结果表明:与新鲜豆油组相比,氧化豆油组肌肉滴水损失和丙二醛MDA含量显著增加(p <0.05);肌肉内还原型谷胱甘肽(GSH)含量、总超氧化物歧化酶(T-SOD)活力和肌肉总抗氧化能力(T-AOC)显著降低(p <0.05);腿肌肌肉中γ-谷氨酰半胱氨酸合成酶调节亚单位(γ-GCLm)和γ-谷氨酰半胱氨酸合成酶催化亚单位(γ-GCLc)的mRNA表达量显著降低(p <0.05)。与不含BHT日粮组相比,日粮中添加125 mg/kg BHT能够显著降低肌肉中MDA含量、肌肉滴水损失及蒸煮损失(p <0.05),能够显著提高肌肉T-SOD和CAT酶活(p <0.05),显著提高肌肉中CAT和γ-GCLm的mRNA表达量(p <0.05)。结论:日粮中添加BHT能够通过提高黄羽肉鸡机体抗氧化能力,改善肉品质,缓解氧化豆油造成的氧化损伤。The study was conducted to evaluate the effects of butylated hydroxytoluene (BHT) on meat quality muscle antioxidant capacity of yellow broilers fed oxidized soybean oil.2 × 2 factorial experimental design was used in our research.Factor 1 was the soybean oil in the diet,according to its quality divided into fresh soybean oil (MDA = 13.4 nmol/mL) and oxidized soybean oil (MDA = 367.7 nmol/mL).Factor 2 was the BHT content in the diet,according to the amount of additives divided into two levels (0,125 mg/kg).240 one-day-old fine quality yellow broilers were randomly divided into 4 treatment groups with 6 replicates in each group and 10 chickens in each replicate.The results showed that compared with fresh soybean oil,oxidized soybean oil could significantly increase the drip loss and MDA content of the muscle (p < 0.05). And oxidized soybean oil could also significantly decrease the glutathione (GSH) content,total superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC) of the muscle (p < 0.05).And the mRNA expression of γ-modifier subunit of glutamatecysteine ligase (γ-GCLm) and γ-catalytic subunit of glutamate-cysteine ligase (γ-GCLc) in thigh muscles were also significantly reduced by oxidized soybean oil (p < 0.05).Compared with the BHT-free diet group,the addition of 125 mg/kg BHT in the diet significantly reduced MDA content in the muscle,muscle drip loss and cooking loss (p < 0.05),and significantly increased muscle T-SOD and CAT activity.(p < 0.05),significantly increased mRNA expression of CAT and γ-GCLm in muscle (p < 0.05).Adding BHT to the diet can improve yellow broilers’ antioxidant capacity,improve the muscle quality and alleviate the oxidative damage caused by oxidized soybean oil.

关 键 词：2 6-二叔丁基-4-甲基苯酚(BHT) 氧化豆油 黄羽肉鸡 肉品质 抗氧化能力 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]