中风1号对急性脑出血大鼠磷脂酰肌醇3-激酶/蛋白激酶B信号通路影响  

Effect of Zhongfeng No.1 on Phosphatidylinositol 3-kinase/Protein Kinase B(PI3K/AKT)Signaling Pathway in Rats with Acute Cerebral Hemorrhage

作　　者：梁群[1] 孙德阳 杨洋[2] 朱永志[1] LIANG Qun;SUN Deyang;YANG Yang;ZHU Yongzhi(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《辽宁中医药大学学报》2019年第3期5-8,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81774276);哈尔滨市科技创新人才研究专项资金项目(2017RAXXJ037);黑龙江中医药大学科研基金项目(2017XY03);黑龙江中医药大学双一流及优势特色学科建设项目(051022);朱永志全国名中医药专家传承工作室建设项目

摘　　要：目的:观察中风1号汤剂对急性脑出血大鼠磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/AKT)信号通路的影响。方法:将50只雄性Sprague-Dawley大鼠随机分为5组(中风1号组、甘露醇组、模型组、假手术组、空白组),每组10只,并给予造模,应用荧光定量RT-PCR技术检测各组脑出血大鼠脑组织中磷脂酰肌醇3-激酶和蛋白激酶B的表达水平,运用2-△△CT法分析结果。结果:中风1号组PI3K和AKT相关表达水平相对于模型组显著升高(P<0.05)。结论:中风1号汤剂在急性脑出血时对组织起到的脑保护作用与其激活了PI3K/AKT信号通路有关。Objective:To observe the effect of decoction of Zhongfeng No.1 on phosphatidylinositol 3-kinase/protein kinase B(PI3 K/AKT)signaling pathway in rats with acute cerebral hemorrhage. Methods:Fifty male Sprague-Dawley rats were randomly divided into 5 groups(Zhongfeng No.1 group,mannitol group,model group,sham operation group,and blank group)with 10 rats in each group. RT-PCR was used to detect the expression of phosphoinositide 3-kinase and protein kinase B in the brains of rats with intracerebral hemorrhage. The results were analyzed by 2^-△△CT. Results:The expression levels of PI3 K and AKT in Zhongfeng No.1 group were significantly increase from those in the model group(P<0.05). Conclusion:The brain protective effect of Zhongfeng No.1 Decoction on acute cerebral hemorrhage is related to its activation of PI3 K/AKT signaling pathway.

关 键 词：中风1号 脑出血 聚合酶链式反应 PI3K/AKT 

分 类 号：R743.34[医药卫生—神经病学与精神病学]