miR-199a通过靶向CTGF抑制结直肠癌细胞增殖和迁移  

miR-199a inhibited colorectal cancer proliferation and migration via targeting CTGF

作　　者：周懿[1] 郑浩 杨远 洪永刚 郝立强 林辉[1] ZHOU Yi;ZHENG Hao;YANG Yuan;HONG Yong-gang;HAO Li-qiang;LIN Hui(First Department of Surgery, Shidong Hospital, Shanghai 200438, China;Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China;Second Department of Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200438, China)

机构地区：[1]上海市杨浦区市东医院普外一科,200438 [2]海军军医大学第三附属医院肝外三科,上海200438 [3]海军军医大学第一附属医院普外二科,上海200438

出　　处：《中国医药生物技术》2019年第2期142-148,共7页Chinese Medicinal Biotechnology

基　　金：上海市杨浦区科学技术委员会及卫生和计划生育委员会立项资助(YP18M05);上海市杨浦区重点学科计划+胃肠道肿瘤微创专科(YP16ZA05)

摘　　要：目的旨在探讨miR-199a在结直肠癌(CRC)发生过程中的分子机制。方法用miR-199a模拟物转染至CRC细胞系。通过MTT和Transwell实验鉴定CRC细胞的增殖和迁移能力。Western blot和qRT-PCR检测相关基因的表达水平。双荧光素酶法报告实验鉴定miR-199a与结缔组织生长因子(CTGF)的作用关系。结果 miR-199a在CRC细胞系中下调表达,过表达miR-199a可以显著抑制CRC的上皮间质转化(EMT)和血管生成。CTGF是miR-199a的直接靶点。过表达CTGF可抵消miR-199a对EMT和血管生成的抑制作用。结论 miR-199a通过直接靶向CTGF抑制CRC细胞的EMT和血管生成。Objective microRNAs are expressed abnormally in colorectal cancer (CRC) and could participate in its development. In the study, we aimed to explore the molecular mechanisms of miR-199a in the genesis of CRC. Methods CRC cell lines were transfected with miR-199a mimics. Proliferation and migration were identified by MTT assay and Transwell assay, respectively. Protein and mRNA expression levels of associated genes were measured by Western blot and qRT-PCR, respectively. Dual luciferase assay was used to determine the relation of miR-199a and CTGF. Results miR-199a was down-regulated in CRC cell lines and over expression of miR-199a significantly inhibited EMT and angiogenesis. CTGF was a direct target of miR-199a. Up regulation of CTGF level sufficiently reversed the suppression effects of miR-199a on EMT and angiogenesis. Conclusion miR-199a directly targets CTGF and inhibits its expression, leading to suppression on EMT and angiogenesis of CRC cells. miR-199a might be used as potential therapeutic strategy for CRC treatment.

关 键 词：结直肠肿瘤 上皮-间质转化 结缔组织生长因子 miR-199a 

分 类 号：R735.34[医药卫生—肿瘤]