山西地区HPV流行状况及病毒载量在宫颈病变中的应用研究  

Application of HPV prevalence and viral load in cervical lesions in Shanxi

作　　者：李雪娇 王炜[1] 郝崇华[1] 赵志敏[1] 张建敏[1] Li Xuejiao;Wang Wei;Hao Chonghua;Zhao Zhimin;Zhang Jianmin(Department of Laboratory Medicine,Shanxi Provincial People’s Hospital,Taiyuan 030012,China)

机构地区：[1]山西省人民医院检验科,太原030012

出　　处：《中国实用医刊》2018年第24期14-16,共3页Chinese Journal of Practical Medicine

摘　　要：目的研究山西地区人乳头瘤病毒(HPV)流行率,探讨HPV病毒载量与宫颈癌进展程度之间的关系。方法采用实时荧光定量PCR技术检测HPV各型DNA拷贝数,对HPV 16、HPV 18型阳性患者宫颈细胞进行液基薄层细胞检测(TCT),并进行阴道细胞学分级(TBS)。结果本研究共纳入15 518例女性,其中3 199例(20.61%)检测为HPV阳性;山西地区最常见的HPV类型是HPVl6、HPV52和HPV58型。HPV16型normal/ LSIL/CIN1组、CIN 2/CIN 3组、宫颈癌(CC)组病毒载量分别为3.13(2.48~3.74)、4.48(4.10~5.14)、5.17(4.41~5.63)log copies/104上皮细胞,CIN2/CIN 3组病毒载量高于normal/ LSIL/CIN1组(P<0.01),但低于CC组(P<0.01);HPV18型三组病毒载量分别为4.10(2.86~4.88)、3.90(2.93~5.31)、3.94(3.57~4.73)log copies/104上皮细胞,三组间比较差异未见统计学意义(P=0.983)。结论宫颈病灶组织中HPVI6亚型病毒载量与宫颈病变相关,病变单位细胞内的HPV病毒载量随宫颈病变程度增加而升高,而HPV18亚型病毒载量与宫颈病变程度无关。Objective To study the prevalence of human papillomavirus (HPV) in Shanxi province, and to investigate the relationship between HPV viral load and cervical cancer progression. Methods Real time fluorescence quantitative PCR was used to detect the copy number of HPV DNA copies, the cervical cells of HPV 16 and HPV 18 positive patients were detected by thin-layer cytologic test (TCT), the Bethesda system wasperformed. Results A total of 15 518 women were enrolled in our study, 3 199 (20.61%) of them were identified as positive. The most common HPV types were HPV l6, HPV52 and HPV58. The viral loads of normal/LSIL CIN1, 2/3 CIN CIN and cervical cancer (CC) group for HPV16 were 3.13 (2.48 - 3.74), 4.48 (4.10 - 5.14), 4.10 (4.41 - 5.63) log copies / 104 cells, the viral load of CIN2 / CIN 3 group was higher than that of the normal/LSIL/CIN1 group (P<0.01), but was lower than that of CC group (P<0.01). The viral load for HPV18 were 4.10 (2.86-4.88), 3.90 (2.93-5.31), and 3.94 (3.57-4.73) log copies/104 cells respectively, and there was no significant difference (P=0.983) among the three groups. Conclusions HPVI6 subtype viral load in cervical lesions is correlated with cervical lesions, the HPV viral load in the unit cells of the lesion increase with the increase of cervical lesions, the load of HPV18 subtype is not related to the degree of cervical lesions.

关 键 词：人乳头瘤病毒 病毒载量 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]