长链非编码RNA HOST2对胰腺癌细胞增殖迁移和侵袭的影响  

Effects of long non-coding RNA HOST2 on proliferation, migration and invasion in pancreatic cancer cells

作　　者：陈伟业 邢宏松 江帆[1] 吴国俊[1] 黎建军[1] 孙权[2] 潘德锐[3] CHEN Weiye;XING Hongsong;JIANG Fan;WU Guojun;LI Jianjun;SUN Quan;PAN Derui(Department of General Surgery,Puren Hospital,Wuhan University of Science and Technology,Wuhan 430081,China;Department of General Surgery,Zhongnan Hospital,Wuhan University,Wuhan 430071,China;Surgery Teaching and Research Section,Puren Hospital,Wuhan University of Science and Technology,Wuhan 430081,China)

机构地区：[1]武汉科技大学附属普仁医院普通外科,湖北武汉430081 [2]武汉大学中南医院普通外科,湖北武汉430071 [3]武汉科技大学附属普仁医院外科教研室,湖北武汉430081

出　　处：《中国普通外科杂志》2019年第3期285-291,共7页China Journal of General Surgery

摘　　要：目的:探讨长链非编码RNA(lncRNA)HOST2对胰腺癌细胞增殖、迁移和侵袭的影响及机制。方法:qRT-PCR检测正常胰腺上皮细胞系HPDE6-C7及胰腺癌细胞系Panc-1、AsPC-1、BxPC-3、HPAC中lncRNAHOST2表达水平。将Panc-1细胞分别转染siRNA-HOST2(si-HOST2组)和阴性对照序列(阴性对照组)后,用MTT法测定细胞增殖,细胞划痕和Transwell实验测定细胞迁移和侵袭,Westernblot测定上皮-间质转化(EMT)相关蛋白vimentin、Snail、Twist的表达。以无转染的Panc-1细胞为空白对照组。结果:与正常胰腺上皮细胞HPDE6-C7相比,lncRNAHOST2在各胰腺癌细胞系中的表达均明显上调(均P<0.05)。与空白对照组比较,si-HOST2组细胞增殖、迁移和侵袭能力均明显减弱,vimentin、Twist1、Snail蛋白相对表达量均明显下调(均P<0.05),而阴性对照组上述指标均无明显变化(均P>0.05)。结论:lncRNAHOST2在胰腺癌细胞中高表达,且与胰腺癌增殖、迁移和侵袭密切相关,其机制可能与调节EMT相关蛋白的表达有关。Objective:To investigate the effect of long non-coding RNA (lncRNA) HOST2 on proliferation,migration and invasion in pancreatic cancer cells and the mechanism.Methods:The lncRNA HOST2 expressions in normal pancreatic epithelial cell line HPDE6-C7 and four different pancreatic cancer cell lines (Panc-1,AsPC-1,BxPC-3 and HPAC) were determined by qRT-PCR.Panc-1 cells were transfected with siRNA-HOST2 (si-HOST2 group) or scrambled sequence (negative control group),and then,the proliferative,migration and invasion abilities were analyzed by MTT assay,wound scratch assay and Transwell assay,and the expressions of epithelial–mesenchymal transition (EMT) associated proteins vimentin,Snail and Twist1 were measured by Western blot,respectively.The untransfected Panc-1 cells were used as blank control group.Results:The expressions of lncRNA HOST2 were significantly increased in all the studied pancreatic cancer cell lines compared with HPDE6-C7 cells (all P<0.05).Compared with blank control group,the proliferative,migration and invasion abilities were all significantly reduced,and the expression levels of vimentin,Snail and Twist1 were all significantly decreased in si-HOST2 group (all P<0.05),while all above parameters showed no significant changes in negative control group (all P>0.05).Conclusion:lncRNA HOST2 expression is up-regulated in pancreatic cancer cells,which is closely related to the proliferation,migration and invasion of pancreatic cancer,and its mechanism may be responsible for regulating the expressions of EMT-associated proteins.

关 键 词：胰腺肿瘤 RNA 长链非编码 细胞增殖 肿瘤侵润 上皮-间质转化 

分 类 号：R735.9[医药卫生—肿瘤]