癫痫持续发作时间与大鼠海马苔藓纤维发芽程度及自发性痫性发作的关系  

Relationship between duration of epileptic seizures and mossy fiber sprouting of hippocampal and spontaneous seizures in rats with epilepsy

作　　者：王艺蓉 欧阳青蓉 余巨明[1] 张小东[1] Wang Yirong;Ouyang Qingrong;Yu Juming;Zhang Xiaodong(Department of Neurology,the Affiliated Hospital of North Chuanbei Medical College,Sichuang 637000,China)

机构地区：[1]川北医学院附属医院神经内科,四川637000

出　　处：《脑与神经疾病杂志》2019年第4期203-206,共4页Journal of Brain and Nervous Diseases

基　　金：四川省教育厅科研项目重点项目(2005A085)

摘　　要：目的探讨癫痫持续状态(SE)发作时间与致痫大鼠海马苔藓纤维发芽(MFS)程度及自发性痫性发作的关系。方法 104只雄性成年SD大鼠,随机分为对照组和3个SE实验组,建立氯化锂-重复低剂量匹罗卡品致痫大鼠模型;诱发SE30min(A组)、60min(B组)、90min(C组)后注射水合氯醛终止发作。各组大鼠自SE终止发作后于相同实验条件下普通饲养45d,观察大鼠行为及脑电图(EEG)的变化,记录自发性痫性发作的发生率。通过苏木精-伊红染色、Nissl染色和Timm硫化银组织化学染色方法观察各实验组海马MFS情况。结果氯化锂-重复低剂量匹罗卡品成功诱导大鼠SE的发生,发作程度均达Ⅳ级以上,EEG类似人类颞叶癫痫。80%的大鼠癫痫持续状态均发展为自发痫性发作,与SE时间无关。与对照组相比,实验A、B、C三组双侧海马CA3区均表现MFS(P<0.05)。实验B组与A、C组相比,CA3区MFS明显增加(P<0.05)。结论氯化锂-重复低剂量匹罗卡品可诱导SE,癫痫持续发作60min后终止的大鼠海马CA3区MFS明显增加,SE发作时间与海马MFS程度并不一定呈正相关。Objective To investigate the relationship between the onset time of status epilepticus(SE)and the degree of mossy fiber sprouting(MFS)in hippocampal and spontaneous seizures in rats with epilepsy.Method Totally 104 male adult healthy Sprague-Dawley(SD)rats were randomly divided into control group and epilepsy continuous state model groups to establish epilepsy rat model with lithium chloride-repeated low-dose pilocarpine.In the experimental group,the onset of status epilepticus 30 minutes(group A),60 minutes(group B)and 90 minutes(group C)was terminated by hydrated chloral injection.The rats in each group were raised for 45 days under the same experimental conditions after status epilepticus termination,and the changes of behavior and electroencephalogram(EEG)were observed and the incidence of spontaneous seizures was recorded.The hippocampal mossy fiber sprouting of each experimental group were observed by Hematoxylin-eosin staining,Nissl staining and Timm's silver sulfide histochemistry staining.Results Lithium chloride-repeated low-dose pirocapine can induce status epilepticus,the extent ofⅣlevel or above,and electroencephalogram is similar to human temporal lobe epilepsy.Eighty percent of the rats developed spontaneous epileptic seizures,regardless of the onset time of status epilepticus.Mossy fiber sprouting in CA3 area of bilateral hippocampus in the three groups of experiment A,B and C compared to the control group(P<0.05).Compared with group A and group B,the mossy fiber sprouting in CA3 area was significantly increased of experiment B(P<0.05).Conclusion Lithium chloride-repeated low-dose pilocarpine can induce status epilepticus,mossy fiber sprouting in the hippocampal CA3 area of rats terminated after 60 minutes of epileptic seizures increased significantly,and the onset time of status epilepticus was not necessarily positively correlated with hippocampal mossy fiber sprouting.

关 键 词：癫痫持续状态 苔藓纤维发芽 自发性痫性发作 

分 类 号：R742.1[医药卫生—神经病学与精神病学]