MscL通道在生物纳米技术领域的应用研究  

Application of MscL channel in bionanotechnology

作　　者：洪朗 李月舟 HONG Lang;LI Yue-zhou(Medical School,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学医学院,杭州310058

出　　处：《生物学杂志》2019年第2期78-82,共5页Journal of Biology

基　　金：科技部973计划(No.2014CB910302)

摘　　要：大电导机械敏感性离子通道(Mechanosensitive channel of large conductance, MscL)是细菌上的一种机械敏感性离子通道,起到紧急释放阀门的作用,避免细菌在外界渗透压剧烈下降时破裂死亡。MscL开放口径大,易于修饰、突变和表达,重构于脂质体上仍有活性,是生物纳米技术领域良好的工具分子。近年来MscL在生物纳米技术领域的应用已有大量成果,研究发现通过修饰、突变后的MscL蛋白可以作为纳米给药系统上的分子开关,具有通透孔径和电荷的选择性,并受到光、pH及磁场等环境因素调控。对MscL在生物纳米技术领域的应用研究进行综述。The mechanosensitive channel of large conductance(MscL)functions as an emergency valve in bacteria responding to changes in membrane tension and preventing lysis under acute osmotic shock.Taking the advantage of large pore size,tractable manipulation,and retainment of its mechanosensitivity when reconstituted into lipid bilayer,MscL could be developed into a ideal bio-nano tool.MscL based nanotechnology has advanced recently.These findings demonstrate that MscL channels with modified pore size or ion selectivity could be controlled by light,pH and magnetic field,and could be used for targeted drug release or other nanodevices.In this review,we focus on the progress of MscL based applictions in the field of bionanotechnology.

关 键 词：MSCL 生物纳米技术 分子开关 

分 类 号：Q41[生物学—生理学] R318[医药卫生—生物医学工程]