蛹虫草高产虫草素复合诱变育种及固态发酵优化  

Screen high-producing cordycepin by Cordyceps militaris through composite mutagenesis and optimizaation of solid state fermentation

作　　者：孙翠[1] 王钰[1] 李阔阔 陈达伟[1] SUN Cui;WANG Yu;LI Kuo-kuo;CHEN Da-wei(School of Resources and Environmental Engineering,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601

出　　处：《生物学杂志》2019年第2期103-106,117,共5页Journal of Biology

基　　金：安徽省科技攻关项目(11010302109)

摘　　要：已成功选育出1株高产虫草素诱变菌株C1-7,以蛹虫草C-8为出发菌株,采用^(60)Coγ-UV复合诱变,并通过Design-expert法对其固态发酵条件进行优化分析。结果表明,前期温度为24℃、后期温度为21℃、初始含水量为64%的条件下,虫草素的产量最高可达9.595 mg/g,相对于出发菌株提高132.9%。通过12代传代培养后,遗传稳定性良好,并且通过栽培得到诱变菌株子实体虫草素产量比出发菌株提高了100.2%,该菌株具有潜在的应用价值。A cordycepin high-yielding mutants had been screened.The original strain Cordyceps militaris producing cordycepin was treated with 60 Co-γ-UV irradiation and its fermentation conditions were optimized for cordycepin production.The results showed that solid state fermentation conditions were initial temperature 24℃,late temperature 21℃.Under these conditions,the yield of cordycepin reached 9.595 mg/g,which was 132.9%higher than that of original strains.The strain was continuously sub-cultured to the 12 generation and remained steady after subculture,in addition,100.2%was improved by cultivation of the mutagenesis strain.In conclusion,a high cordycepin-producing strain C1-7 was successfully screened,which has potential application value in practice.

关 键 词：虫草素 复合诱变 蛹虫草 响应面 

分 类 号：Q933[生物学—微生物学]