β-葡萄糖苷酶抗体的制备及比较分析  

Preparation and Comparative Analysis of β-glucosidase Antibodies

作　　者：余厚美[1] 张振文[1] YU Houmei;ZHANG Zhenwen(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/National R&D Centre for Potato Processing/ Ministry of Agriculture and Rural Affairs Key Laboratory for Conservation and Utilization of Cassava Germplasm Resources,Danzhou,Hainan 571737)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/国家薯类加工技术研发分中心/农业部木薯种质资源保护与利用重点实验室,海南儋州571737

出　　处：《热带生物学报》2019年第1期89-93,共5页Journal of Tropical Biology

基　　金：海南省重点研发专项对外合作项目(ZDYF2017157);现代农业产业技术体系(CARS-12)专项资金共同资助

摘　　要：β-葡萄糖苷酶是一种广泛存在于自然界的水解酶,其含量高低直接关系到水解产物的量。木薯含有大量的生氰糖苷,是β-葡萄糖苷酶的底物之一,被水解后将产生有毒的氰化物,给食品安全带来隐患。为保障食品安全,给木薯及其制品的氰化物含量提供快速检测技术。本研究以A,B,C 3种来源的β-葡萄糖苷酶为抗原免疫Balb/C小鼠,并制备单克隆抗体和做出初步评价。结果表明,A抗原免疫的小鼠融合后获得5株杂交瘤细胞株,效价均达到10~5以上,抗体亚型均为IgG1型,但单克隆抗体与B抗原、C抗原、KLH、BSA均无交叉反应。说明不同来源的β-葡萄糖苷酶免疫小鼠得到的抗体均为特异性抗体。β-glucosidase is a hydrolase which exists widely in nature, and its content is directly related to the amount of hydrolysate. Cassava contains a large number of cyanogenic glycosides which are one of the substrates of β-glucosidase and will produce a poisonous substance, cyanide after hydrolyzed by β-glucosidase, bringing hidden dangers to the food safety. For food safety, an attempt was made to provide a rapid detection technique for cyanide content of cassava and its products. The Balb/C mice were immunized with β-glucosidase from A, B and C sources as antigens, and their monoclonal antibodies were prepared and evaluated. The results showed that the antibodies obtained from the mice immunized with the three sources(A, B and C) of β-glucosidase did not cross-react with each other. Five hybridoma cell lines were obtained from the spleen of the mice immunized with Antigen A, and the titer of antibodies was above 10~5. All the subtypes were IgG1, but all the monoclonal antibodies had no cross-reaction with the Antigens B and C, KLH and BSA. It was concluded that the antibodies obtained from the mice immunized with different sources of β-glucosidase were specific.

关 键 词：Β-葡萄糖苷酶 抗体 抗体制备 

分 类 号：Q-331[生物学]