急性缺血性脑卒中大鼠脑组织中Th17/Treg的变化  

Change of Th17/Treg in rat brain with acute ischemic stroke

作　　者：程建华[1] 黄一睿 肖美娟[1] 韩钊[3] CHENG Jian-hua;HUANG Yi-rui;XIAO Mei-juan;HAN Zhao(Department of Neurology, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000 , China;Clinical Laboratory of Wenzhou Integrated Traditional Chinese and Western Medicine Hospital, Wenzhou 325000 , China;Department of Neurology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000 , China)

机构地区：[1]温州医科大学附属第一医院神经内科,浙江温州325000 [2]温州市中西医结合医院检验科,浙江温州325000 [3]温州医科大学附属第二医院神经内科,浙江温州325000

出　　处：《中国病理生理杂志》2019年第4期758-763,768,共7页Chinese Journal of Pathophysiology

基　　金：浙江省自然科学基金资助项目(No.LY17H090014);温州市科技局科技计划项目(No.Y20170339)

摘　　要：目的:免疫炎症反应在急性缺血性脑卒中的病理生理过程中发挥着重要的作用。具有促炎作用的辅助性T细胞17(Th17)及维持免疫耐受的调节性T细胞(Treg)是体内重要的2种免疫细胞。Th17/Treg细胞平衡是机体维持正常免疫的基础。本研究探讨急性缺血性脑卒中大鼠脑组织中Th17/Treg的变化。方法:采用线栓法制备SD大鼠急性大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,以假手术组作为对照组。在MCAO术后3 d利用TTC染色观察各组大鼠脑梗死体积;采用ELISA测定脑组织中白细胞介素17A(IL-17A)和IL-10蛋白的含量;采用RT-qPCR测定脑组织中IL-17、IL-10、Foxp3和RORγt的mRNA表达水平;采用流式细胞术测定脑组织中Th17细胞和Treg细胞的比例变化。结果:与假手术组相比,MCAO组大鼠脑组织中IL-17A的含量增加,IL-10的含量减少(P<0.05);RORγt和IL-17的mRNA表达水平上调(P<0.05),Foxp3和IL-10的mRNA表达水平下调(P<0.05);脑组织Th17细胞增多,Treg细胞明显减少(P<0.05),Th17/Treg比值升高(P<0.05)。结论:急性缺血性脑卒中大鼠脑组织Th17细胞增多,Treg细胞减少,表明脑梗死后大鼠脑组织中Th17/Treg的平衡被破坏,免疫炎症反应被激活。AIM: To investigate the changes of Thelper 17(Th17) cells/regulatory T(Treg) cells in the brain of rats with acute ischemic stroke. METHODS: Acute middle cerebral artery occlusion(MCAO) model was established by thread embolization in SD rats, and sham operation was used as control. The infarct volume of the rats in each group was observed by TTC staining on day 3 after MCAO. The levels of interleukin-17 A(IL-17 A) and IL-10 in the brain tissues were measured by ELISA. RT-qPCR was used to detect the mRNA levels of IL-17, IL-10, Foxp3 and RORγt. The proportion of Th17 cells and Treg cells was measured by flow cytometry analysis. RESULTS: Compared with sham operation group, the protein level of IL-17 A in MCAO group was increased(P<0.05), and the protein level of IL-10 was decreased(P<0.05). The mRNA expression of RORγt and IL-17 was increased(P<0.05), and the mRNA levels of Foxp3 and IL-10 were decreased(P<0.05). The proportion of Th17 cells was increased(P<0.05), while the proportion of Treg cells was decreased(P<0.05), and the Th17/Treg ratio was thus increased(P<0.05). CONCLUSION: The increase in Th17 cells and the decrease in Treg cells in the brain tissues of acute ischemic rats indicate that the immuno-inflammatory response is activated after cerebral infarction, and the balance of T helper 17/Treg is broken.

关 键 词：急性缺血性脑卒中 免疫 炎症 辅助性T细胞17 调节性T细胞 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R363.2[医药卫生—临床医学]