De?ning gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen  

作　　者：Yuan Gao Ying Mao Rong-Gang Xu Ruibao Zhu Ming Zhang Jin Sun Da Shen Ping Peng Ting Xie Jian-Quan Ni 

机构地区：[1]PKU-THU Joint Center for Life Sciences,College of Life Sciences,School of Medicine,Tsinghua University,Beijing,100084,China [2]Gene Regulatory Lab,School of Medicine,Tsinghua University,Beijing,100084,China [3]Stowers Institute for Medical Research,1000 East 50th Street,Kansas City,MO,64110,USA [4]Tsingdao Advanced Research Institute,Tongji University,Qingdao,266000,China

出　　处：《Journal of Genetics and Genomics》2019年第1期19-30,共12页遗传学报（英文版）

基　　金：supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People's Republic of China (2016YFE0113700);the National Natural Science Foundation of China (31571320, 31872818, and 31801079)

摘　　要：In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC) maintenance and progeny differentiation. However, the underlying mechanisms remain poorly understood. In this study,we identified 173 EC genes for their roles in controlling GSC maintenance and progeny differentiation by using an in vivo systematic RNAi approach. Of the identified genes, 10 and 163 are required in ECs to promote GSC maintenance and progeny differentiation, respectively. The genes required for progeny differentiation fall into different functional categories, including transcription, mRNA splicing, protein degradation, signal transduction and cytoskeleton regulation. In addition, the GSC progeny differentiation defects caused by defective ECs are often associated with BMP signaling elevation, indicating that preventing BMP signaling is a general functional feature of the differentiation niche. Lastly, exon junction complex (EJC) components, which are essential for mRNA splicing, are required in ECs to promote GSC progeny differentiation by maintaining ECs and preventing BMP signaling. Therefore, this study has identified the major regulators of the differentiation niche, which provides important insights into how stem cell progeny differentiation is extrinsically controlled.In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC) maintenance and progeny differentiation. However, the underlying mechanisms remain poorly understood. In this study,we identified 173 EC genes for their roles in controlling GSC maintenance and progeny differentiation by using an in vivo systematic RNAi approach. Of the identified genes, 10 and 163 are required in ECs to promote GSC maintenance and progeny differentiation, respectively. The genes required for progeny differentiation fall into different functional categories, including transcription, mRNA splicing, protein degradation, signal transduction and cytoskeleton regulation. In addition, the GSC progeny differentiation defects caused by defective ECs are often associated with BMP signaling elevation, indicating that preventing BMP signaling is a general functional feature of the differentiation niche. Lastly, exon junction complex (EJC) components, which are essential for mRNA splicing, are required in ECs to promote GSC progeny differentiation by maintaining ECs and preventing BMP signaling. Therefore, this study has identified the major regulators of the differentiation niche, which provides important insights into how stem cell progeny differentiation is extrinsically controlled.

关 键 词：GERMLINE stem CELLS ESCORT CELLS In VIVO SCREEN 

分 类 号：R[医药卫生]