IL-22基因单核苷酸多态性与结直肠癌易感性和临床病理参数的相关性  

Expression of single nucleotide polymorphisms of interleukin 22 in colorectal cancer: correlations with susceptibility and clinicopathological parameters

作　　者：叶石才 郑学宝 朱朱[1] 周宇 钟宇 黄哲 王永存 叶华 邹颖 冯锦山 迟宏罡 朱宇珍 Ye Shicai;Zheng Xuebao;Zhu Zhu(Guangdong Medical University,Zhanjiang 524000;Guangzhou University of Chinese Medicine,Guangzhou 510006)

机构地区：[1]广东医科大学,湛江524000 [2]广州中医药大学,广州510006

出　　处：《安徽医科大学学报》2019年第4期617-621,共5页Acta Universitatis Medicinalis Anhui

基　　金：广东省医学科研基金立项项目(编号:A2017005;B2017414;B2017044;A2018495);广东省普通高校省级重大科研项目(编号:2017KZDXM017);国家自然科学基金(编号:81573932;81603592);广东省科技发展专项资金(基础与应用基础研究方向)项目(编号:2016A030310356);湛江市科技计划项目(编号:2014A01014);广东医科大学科研基金面上培育项目(编号:M2016021);广东省中医药局科研项目(编号:20182072;20182069)

摘　　要：目的探讨白细胞介素22(IL-22)基因单核苷酸多态性与结直肠癌易感性和临床病理参数的相关性。方法选取结直肠癌患者306例,健康对照者358例,采用TaqMan探针技术,对IL-22基因两个SNP位点(rs2227473和rs2227483)进行基因分型,统计分析结直肠癌患者和健康对照者的基因频率,并结合结直肠癌患者不同的临床特点、病理特征进行分层比较。结果 IL-22基因rs2227473位点在结直肠癌患者中G的等位基因频率为92%,在健康对照者中的等位基因频率为85.6%,差异具有统计学意义(χ~2=13.25,P=0.000 3);在临床分期中,rs2227473位点G的在Ⅰ+Ⅱ期患者中的基因频率为80.3%,在Ⅲ+Ⅳ期患者中的基因频率为91.5%,差异具有统计学意义(χ~2=16.7,P<0.000 1)。而rs2227473位点的基因型与血清癌胚抗原(CEA)、原发肿瘤扩展情况、淋巴结状态和转移情况的参数之间无显著相关性。且rs2227483位点的基因型及等位基因频率分布在结直肠癌患者与健康对照者间差异有统计学意义。结论 IL-22基因rs2227473多态性与结直肠癌的易感性和临床分期相关,其中携带等位基因G增加结直肠癌的发生风险。Objective To investigate the expression of single nucleotide polymorphisms(SNPs)of interleukin 22(IL-22)in colorectal cancer:correlations with susceptibility and clinicopathological parameters.Methods A total of 306 patients with colorectal cancer and 358 healthy controls were enrolled.The two SNPs(rs2227473 and rs2227483)were genotyped using TaqMan probe technique.The results were statistically analyzed for the gene frequency of colorectal cancer patients and healthy controls,and combined with different clinical features and pathological features of colorectal cancer patients for stratified comparison.Results The genotypic frequency of G of IL-22(rs2227473)in the patient groups(92%)were significantly higher than that of the control groups(85.6%)(χ^2=13.25,P=0.000 3).In clinical stage,the gene frequency of rs2227473 site G in patients with stageⅠ+Ⅱwas 80.3%,while in stageⅢ+Ⅳwas 91.5%.There was no significant correlation between rs2227473 and the parameters of serum carcinoembryonic antigen(CEA),primary tumor expansion,lymph node status and metastasis.The genotypic frequency of IL-22(rs2227483)was not statistically different between colorectal cancer patients and healthy controls.Conclusion The polymorphism of IL-22(rs2227473)gene may be associated with the susceptibility and clinical stage of colorectal cancer.G allele gene may be related to the risk of colorectal cancer.

关 键 词：白细胞介素-22 结直肠癌 基因多态性 遗传易感性 临床病理 

分 类 号：R735.3[医药卫生—肿瘤]