小檗碱调控TLR4/NF-κB信号通路抑制宫颈癌Hela细胞增殖研究  

Effects of berberine on proliferation of Hela cells through TLR4-NF-κB pathway

作　　者：洪丹丹[1] 任青玲[1] 徐传花[1] 徐雯雯[1] 王伟[1] 荣慧 郭红玉[1] 赵玉芹[1] 马蔚蓉[1] 曹丹丹 陈冰倩 HONG Dandan;REN Qingling;XU Chuanhua;XU Wenwen;WANG Wei;RONG Hui;GUO Hongyu;ZHAO Yuqin;MA Weirong;CAO Dandan;CHEN Bingqian(Department of Gynecology,First Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210000,China)

机构地区：[1]南京中医药大学第一附属医院妇科,南京210000

出　　处：《实用医学杂志》2019年第5期692-697,共6页The Journal of Practical Medicine

基　　金：江苏省中医院科技项目(编号:Y2017CX14);第二周期省妇幼健康重点人才项目(省卫生计生委)(编号:FRC201711)

摘　　要：目的探讨TLR4-NF-κb信号通路在小檗碱发挥抑制HeLa细胞增殖中的作用。方法分别给予5、10和40μg/mL 3种不同剂量干预Hela细胞,并通过特异性siRNA干扰TLR4表达。通过RT-qPCR和Western法检测TLR4、p65和Bcl-2的mRNA和蛋白表达改变,通过MTT法、Transwell小室和TUNEL评价细胞生长、迁移和凋亡情况。结果软件设计+RT-qPCR确定转染抑制效率最高的siRNA-002进行后续实验。小檗碱呈浓度依赖性抑制Hela细胞增殖和迁移,促进细胞凋亡,抑制p65、Bcl-2和TLR4的mRNA和蛋白表达。加用siRNA-002后,小檗碱的上述作用进一步增强。结论小檗碱可调控TLR4-NF-κB信号通路下调Bcl-2表达,显著抑制宫颈癌Hela细胞的增殖和迁移,促进其凋亡,为小檗碱治疗宫颈癌提供新的理论依据。Objective To evaluate the effects of berberine on the proliferation of Hela cells through TLR4-NF-κB pathway. Methods Three different dose of berberine(5,10 and 40 μg/mL) and special TLR4-siRNA were used to intervene Hela cells. mRNA and protein of TLR4,p65 and Bcl-2 were evaluated by RT-qPCR and Western blot,respectively. The changes of proliferation,m igration and apoptosis of Hela cells were estimated by MTT,Transwell chamber and TUNEL,respectively. Results SiRNA-002 was selected as the strongest silence siR NA by RT-qPCR. The berberine could inhibit the proliferation and migration,promote the apoptosis,down regulate the mRNA and protein of p65,Bcl-2 and TLR4 of Hela cells,which showed a dose dependent effect. The effects of berberine above mentioned were enhanced by siRNA-002. Conclusion Berberine may inhibit Hela cells′ prolifera tion and migration,promote apoptosis by the down regulation of Bcl-2 through TLR4-NF-κB pathway.

关 键 词：小檗碱 Toll样受体4-核因子κB信号通路 宫颈癌细胞 

分 类 号：R737.33[医药卫生—肿瘤]