酸蚀纳米钛合金种植体的早期骨整合效果  

Enhanced osseointegration of nano textured Ti6Al4V implants by etching

作　　者：吴伟力[1] 罗军[1] 应于康 朱海钱[1] 陶丹[1] 徐攀舒 于卫强[2] WU Weili;LUO Jun;YING Yukang;ZHU Haiqian;TAO Dan;XU Panshu;YU Weiqiang(Department of Stomatological,the Central Hospital of Taizhou,Taizhou 318000,China;Department of Prosthodontics,Shanghai Ninth People’s Hospital,Shanghai 200011,China)

机构地区：[1]台州市中心医院口腔科,浙江台州318000 [2]上海第九人民医院口腔修复科,上海200011

出　　处：《温州医科大学学报》2019年第4期285-288,共4页Journal of Wenzhou Medical University

基　　金：台州市科技计划项目(1501KY18)

摘　　要：目的:体内评估用酸蚀法构建的纳米钛合金种植体的旋转扭力和骨功能基因的表达情况。方法:钛合金Ti6Al4V种植体分为对照组(机械切割钛合金种植体)和纳米钛组(酸蚀纳米钛合金种植体)。常规将钛合金种植体植入新西兰兔子的胫骨,植入后4周,采用数字扭力仪测试钛合金种植体的旋转扭力值,采用钙黄素染色观测种植体周围的新骨生长情况,并且通过实时定量PCR检测骨功能基因核心结合因子(Runx2)、I型胶原(Col-1)和骨钙素(OCN)的表达水平。结果:纳米钛合金种植体的旋转扭力显著大于机械切割钛合金种植体(P<0.05),荧光染色显示纳米钛合金种植体周围有更多的骨组织长入,并且纳米种植体可以提高Runx2、Col-I和OCN基因的表达(P<0.05)。结论:酸蚀法在钛合金表面形成的纳米结构有利于种植体的早期骨整合,在种植材料领域有一定的应用前景。Objective:To evaluate the removal torque and bone gene expression of nano textured Ti6Al4V implants in vivo.Methods:The implants were divided as group of nano textured Ti6Al4V implants by etching and as the control of the machined implants.All the implants were placed in the tibia of rabbits.After four weeks,removal torque was analyzed by a digital torque gauge.New bone was found with fluorescence microscope.The gene expression of Runx2,Col-1 and OCN was examined using real-time PCR.Results:Nano textured Ti6Al4V implants by etching showed significantly bigger removal torque(P<0.05)and higher Runx2,Col-1 and OCN gene expression(P<0.05)than the machined implants.In addition,more new bone was found around nano textured Ti6Al4V implant around.Conclusion:The nano textured Ti6Al4V implants by etching could improve the early osseointegration processin vivo,which may be applied clinically in the future.

关 键 词：纳米 种植体 骨整合 

分 类 号：R783.1[医药卫生—口腔医学]