贵州草海小龙虾虾青素提取条件及含量测定  

Extraction conditions and content determination of astaxanthin from crayfish in Lake Caohai,Guizhou Province

作　　者：张旭 冉景丞 杨卫诚[1,3] 陶红梅[1,3] 姜莲 ZHANG Xu;RAN Jing cheng;YANG Wei cheng;TAO Hong mei;JIANG Lian(School of life sciences,Guizhou normal university,Guiyang,550000,China;The forestry department of Guizhou Province,Guiyang,550000,China;Karst cave research center,Guizhou normal university,Guiyang,550000,China)

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550000 [2]贵州省林业厅,贵州贵阳550000 [3]贵州师范大学喀斯特洞穴研究中心,贵州贵阳550000

出　　处：《河北渔业》2019年第4期14-18,共5页

基　　金：国家自然科学基金项目(No.30560024);贵州省科技基金项目(黔科合LH字LKS[2016]7207号;黔科合支撑[2017]2811号;黔科合重大专项字[2016]3022-03号);2017年贵州师范大学博士基金项目

摘　　要：以贵州草海自然水域捕获的新鲜小龙虾(Procambarus clarkii)为材料,采用单因素实验研究了提取溶液、料液比、提取温度、提取时间、提取次数对虾青素提取效果的影响,用分光光度法测定了草海小龙虾不同部位虾青素的含量。结果表明,以乙酸乙酯为提取溶液,料液比为1∶2(g∶mL),在60℃下提取2 h,重复提取两次,再此提取条件下虾壳中虾青素含量最高。在虾壳、虾肉和内脏三个部位中,虾壳中虾青素含量最高,虾肉和内脏中也含有少量虾青素,但是含量远低于虾壳中的含量,说明了小龙虾虾青素主要富集在虾壳中。Using fresh crayfish( Procambarus clarkii ) caught in natural waters of Lake Caohai in Guizhou Province as materials,the effects of extraction solution,ratio of material to liquid,extraction temperature,extraction time and extraction times on the extraction efficiency of astaxanthin were studied by single factor experiment.The astaxanthin content in different parts of crayfish was determined by spectrophotometry.The results showed that astaxanthin content in shrimp shells was the highest when ethyl acetate was used as extracting solution and the ratio of material to liquid was 1∶2(g∶mL).The extracting time was 2 hours at 60 ℃,and the extraction was repeated twice.Among the three parts of shrimp shell,shrimp meat and viscera,astaxanthin content in shrimp shell is the highest,and a small amount of astaxanthin is also contained in shrimp meat and viscera,but the content is much lower than that in shrimp shell,which indicates that astaxanthin is mainly concentrated in shrimp shell.

关 键 词：小龙虾(Procambarus clarkii) 虾青素含量 提取工艺 分光光度法 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]