PD-L1和BAFF-R在胃弥漫性大B细胞淋巴瘤中的表达及其临床意义  

Clinical Significance of PD-L1 and BAFF-R Expression in Gastric Diffuse Large B-cell Lymphoma and Adjacent Nontumors Tissues

作　　者：曾辉 王进峰 尚松 王亮 李亚军 李俊军 邓红玉 欧阳琳达 邓日林 唐松青 谢海龙[1] 朱海珍 左朝晖[1,2] ZENG Hui;WANG Jin-feng;SHANG Song;WANG Liang;LI Ya-jun;LIJun-jun;DENG Hong-yu;OUYANG Lin-da;DENG Ri-lin;TANG Song-qing;XIE Hai-long;ZHU Haizhen;ZUO Chao-hui(Graduates School, University of South China, Hengyang 421001, Hunan, China;Department of Gastroduodenal and Pancreatic Surgery, Translational Medicine Research Center of LiverCancer, Laboratory of Digestive Oncology, Affiliated Cancer Hospital of Xiangya Medical School & Hunan Province Cancer Hospital, Central South University, Changsha 410013, Hunan, China;Institute of Pathogen Biology and Immunology of College of Biology, State Key Laboratory of Chemo / Biosensor and Chemometrics, Hunan University, Changsha 410082, Hunan, China)

机构地区：[1]南华大学研究生院,湖南衡阳421001 [2]中南大学湘雅医学院附属肿瘤医院暨湖南省肿瘤医院胃十二指肠胰腺外科湖南省肿瘤医院(湖南省肿瘤防治研究所)肝癌转化医学联合研究中心和消化道肿瘤实验室,长沙410013 [3]湖南大学生物学院病原微生物与免疫学研究所化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《中国现代手术学杂志》2019年第1期1-6,共6页Chinese Journal of Modern Operative Surgery

基　　金：湖南省发展和改革委员会产业研发项目(2015-99);湖南省科技厅重点研发计划项目(2017k2130)

摘　　要：目的探讨程序性死亡蛋白受体-1配体(programmed death ligand-1, PD-L1)和TNF家族的B细胞活化因子(B cell-activating factor belonging to the TNF family, BAFF-R)在胃弥漫性大B细胞淋巴瘤(gastric diffuse large B-cell lymphoma, GDLBCL)的瘤组织和瘤旁组织中的表达及其临床意义。方法采用免疫组织化学法和定量逆转录-聚合酶链反应法(quantitative real time polymerase chain reaction, qRT-PCR)检测26例GDLBCL瘤组织和瘤旁组织中的PD-L1和BAFF-R的表达,分析PD-L1和BAFF-R的表达与GDLBCL临床分期和病理分型之间的关系和PD-L1与BAFF-R表达的相关性。结果 PD-L1和BAFF-R在GDLBCL中的表达显著高于瘤旁组织(P<0.01)。PD-L1和BAFF-R表达与GDLBCL分期及病理分型均有明显相关性(P<0.01),PD-L1与BAFF-R有相关性(P<0.01)。结论 PDL-1和BAFF-R表达上调与GDLBCL分期及病理分型密切相关,联合检测PD-L1和BAFF-R可能成为GDLBCL有价值的预后指标。以PD-L1和BAFF-R信号通路为靶点的治疗有望成为GDLBLC免疫治疗的潜在方案。Objective To explore the clinical significance of programmed death ligand-1(PD-L1) and B-cell activating factor belonging to the TNF family-receptor(BAFF-R) expression in gastric diffuse large B-cell lymphoma(GDLBCL) and para-tumor tissues. Methods The PD-L1 and BAFF-R expression was examined in 26 matched sets of GDLBCL specimens and adjacent tissues using immunohistochemistry and quantificational reverse transcription-polymerase chain reaction( qRT-PCR). It was analyzed of the expession of PDL1 or BAFF-R with tumor stages and other pathological characteristics. And the relation of PD-L1 expression and BAFF-R in GDLBCL was explored as well. Results The PD-L1 and BAFF-R expression was significantly higher in GDLBCL than adjacent non-tumoral tissues( P<0.01). There were significant correlations on PDL1 and BAFF-R expression with tumor stages and pathological classifications( P<0.01),and the PD-L1 expression was correlation with BAFF-R in the GDLBCL( P< 0.01). Conclusions The up-regulated expression of PD-L1 and BAFF-R was highly correlated with the clinical stages or the pathological types of GDLBCL. Examining PD-L1 and BAFF-R expression might be valuable indications for GDLBCL inspection. Targeting PD-L1 and BAFF-R signaling pathway might be a potential immunotherapy schedule for GDLBCL.

关 键 词：胃弥漫性大B细胞淋巴瘤 程序性死亡蛋白-1配体 B淋巴细胞活化因子受体 定量逆转录-聚合酶链反应法 肿瘤免疫治疗 

分 类 号：R735.2[医药卫生—肿瘤]