多粘类芽孢杆菌HK18-8对辣椒炭疽病菌的抑制作用及其定殖能力  

Antifungal Activity of Paenibacillus polymyxa HK18-8 Against Pepper Anthracnose and Its Colonization Ability

作　　者：申顺善[1] 张涛[2] 王娟[1] 刘东平[1] 张珊珊 孙治强[2] 朴凤植[2] SHEN Shunshan;ZHANG Tao;WANG Juan;LIU Dongping;ZHANG Shanshan;SUN Zhiqiang;PIAO Fengzhi(College of Plant Protection, Henan Agricultural University,Zhengzhou 450002, China;College of Horticulture, Henan Agricultural University, Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学园艺学院,郑州450002

出　　处：《园艺学报》2019年第3期499-507,共9页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2017YFD0201100;2016YFD0201008);国家现代农业产业技术体系建设专项资金项目(CARS-25-C-06)

摘　　要：从健康的化香(Platycarya strobilacea Sieb. et Zucc.)根际土壤中分离获得生防菌HK18-8,为探究其对辣椒炭疽病的生防潜力,通过对峙培养和孢子萌发试验发现,HK18-8显著抑制短尖刺盘孢(Collectotrichum acutatum)的菌丝生长和分生孢子萌发,其抑菌带达到10mm,孢子萌发抑制率为70.15%;进行离体辣椒果实接种试验表明,HK18-8菌悬液对辣椒炭疽病的防治效果达到88.58%,其预防效果高于治疗效果,HK18-8的发酵液和菌悬液的防病效果显著;采用平板稀释法测定其在辣椒果实的定殖能力,处理9 d时仍能保持10~6 cfu·g^(-1)以上;根据形态特征、生理生化特性及16S rRNA序列同源性,HK18-8菌株鉴定为多粘类芽孢杆菌(Paenibacillus polymyxa)。A promising biocontrol agent HK18-8,a strain with strong effect on pepper anthracnose,was selected from the rhizosphere of healthy dyetree plant. Its antifungal effect and colonization ability were detected by in vitro experiments. The results showed that,HK18-8 was able to inhibit the mycelial growth and conidia germination of Colletotrichum acutatum significantly in the in vitro experiment. The inhibition zone of the mycelial growth reached to 10 mm,and the inhibition rate of conidia germination was 70.15%. HK18-8 performed the successful potential control against pepper anthracnose(the control efficiency was 88.58%),and the preventive effect was higher than its therapeutic effect. In addition,HK18-8 was readily able to colonize on fruit surface of pepper,persisting with a relative population density of more than 106 cfu · g^-1. Based on the morphological,physiological characteristics and 16 S rRNA sequence analysis,the strain HK18-8 was identified as Paenibacillus polymyxa.

关 键 词：辣椒 多粘类芽孢杆菌 辣椒炭疽病 抑制活性 防治效果 定殖能力 

分 类 号：S641.3[农业科学—蔬菜学]