香烟烟雾提取物对人颌骨来源成骨细胞矿化能力的影响  

Effects of cigarette smoke extracts on mineralization ability of human jawbone-derived osteoblasts

作　　者：张志宏[1] 李钧[2] 王丁[1] 庄润涛 ZHANG Zhihong;LI Jun;WANG Ding;ZHUANG Runtao(Department of Stomatology, Beijing Civil Aviation General Hospital,Beijing 100123, China;Beijing Stomatological Hospital, CapitalMedical University;Community Health Service Center, BeijingJiaotong University)

机构地区：[1]北京民航总医院口腔科,北京100123 [2]首都医科大学附属北京口腔医院 [3]北京交通大学社区卫生服务中心

出　　处：《山西医科大学学报》2019年第4期468-472,共5页Journal of Shanxi Medical University

摘　　要：目的观察香烟烟雾提取物对人颌骨来源成骨细胞体外矿化能力的影响。方法成骨细胞来源于首都医科大学附属北京口腔医院和民航总医院口腔科,选择30位进行牙种植的健康志愿者,并进行分离培养。准备香烟烟雾提取物(cigarette smoke extract,CSE),并在实验中按照提取物的终浓度分为低浓度(0. 5%) CSE组和和高浓度CSE(3%)组,同时设立无香烟烟雾提取物组为阴性对照组。分别用MTT法检测三组细胞的增殖能力,碱性磷酸酶染色法检测人颌骨来源成骨细胞的体外矿化能力,RT-PCR检测各组ALP、BSP和COL-1的表达情况。结果与对照组比较,低浓度CSE组颌骨来源成骨细胞增殖和矿化能力差异无统计学意义(P> 0. 05),同时ALP、COL-1和BSP的表达差异无统计学意义(P> 0. 05);高浓度CSE组颌骨来源成骨细胞的增殖能力、矿化能力均低于对照组和低浓度CSE组(P <0. 05),同时ALP、COL-1和BSP表达低于对照组与低浓度组(P <0. 05)。结论 CSE能够影响成骨细胞矿物质支架的形成和矿物质的沉积,使新骨生成的数量和质量都降低,并降低了成骨细胞细胞外基质的钙化程度,从而影响种植体的骨结合。Objective To observe the effects of cigarette smoke extract on the mineralization ability of human jawbone-derived osteoblasts in vitro . Methods Osteoblasts were obtained from 30 healthy subjects with dental implants in the Department of Stomatology, Beijing Stomatological Hospital and the General Hospital of Civil Aviation,Affiliated to Capital Medical University, and then and isolated. The final concentration of extract was 0.5%(low concentration group) and 3%(high concentration CSE group), and smoke-free extract was used as negative control group. The proliferation ability was detected by MTT assay, the in vitro mineralization ability of human jawbone-derived osteoblasts was detected by alkaline phosphatase staining method, and the expression of ALP, BSP and COL-1 in each group was detected by RT-PCR. Results There was no significant difference in the proliferation and mineralization of jawbone-derived osteoblasts between control group and low-concentration CSE group( P >0.05), and there was also no significant difference in the expression of ALP, COL-1 and BSP( P >0.05). The proliferation and mineralization ability of osteoblasts in high concentration group were lower than those in control group and low concentration CSE group( P <0.05), and the expression of ALP, COL-1 and BSP was also lower than that of control group and low concentration group( P <0.05). Conclusion CSE can affect the formation of osteoblast mineral scaffolds and the deposition of minerals, reduce the quantity and quality of new bone formation, and reduce the degree of calcification of extracellular matrix of osteoblasts, thus affecting the osseointegration of implants.

关 键 词：成骨细胞 烟雾提取物 增殖 矿化能力 种植体 

分 类 号：R783[医药卫生—口腔医学]